Tetrode Implantation
Tetrode Implantation - Related Videos
06:34
A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice
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Cited by 2 •
2023
Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.
15:28
Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects
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Cited by 24 •
2014
We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allows us to monitor activity in individual units of tethered cockroaches. Here we present a modified version of that technique that allows us to also record brain activity in freely moving insects.
14:19
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
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Cited by 111 •
2009
In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.
16:40
A New Single Chamber Implantable Defibrillator with Atrial Sensing: A Practical Demonstration of Sensing and Ease of Implantation
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Cited by 5 •
2012
Dual-chamber implantable cardioverter-defibrillators (ICDs) may improve detection of atrial fibrillation as well as differentiation of tachycardias. However, this advantage is undermined by complications associated with the second electrode, which is required in conventional dual chamber devices. Therefore, BIOTRONIK has developed a new electrode called the LinoxSMART S DX that, when used in conjunction with the Lumax DX ICD, offers dual-chamber detection without the risks associated with the...
05:54
Microbead Implantation in the Zebrafish Embryo
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Cited by 8 •
2015
The zebrafish is an excellent model system for genetic and developmental studies. Bead implantation is a valuable tissue manipulation technique that can be used to interrogate developmental mechanisms by introducing alterations in local cellular environments. This protocol describes how to perform microbead implantation in the zebrafish embryo.
FAQs
The four wires sample extracellular voltage changes at closely spaced locations within the same implanted bundle. A spike from a nearby neuron can therefore produce different signal amplitudes on the individual channels. Comparing those amplitude patterns helps investigators distinguish spikes originating from different cells and classify them as putative neuronal units.
A single tetrode does not rely on one voltage trace alone. Because its four conductors are closely spaced, the same neuronal event appears with a characteristic distribution of amplitudes across channels. That multichannel pattern supplies the basis for separating activity from nearby cells and assigning detected spikes to putative units rather than treating all events as one signal.
A microdrive allows researchers to adjust tetrode position across recording sessions. This flexibility changes where the electrodes sample neural activity while retaining the implanted recording platform. The resulting measurements can help investigators follow firing from individual neurons or populations as behavior is recorded, linking activity to behavioral conditions over time.
The approach places a tetrode bundle into brain tissue, uses its four conductive wires to detect extracellular voltage changes, and relies on a microdrive to position or adjust the bundle. During recording sessions, investigators compare signals across the four channels, then separate detected spikes into putative neuronal units for analysis.
These recordings can examine how individual neurons and neural populations respond during sensory processing, learning, memory, and navigation. The method provides firing activity that can be analyzed in relation to behavior, allowing researchers to investigate coordinated neural responses rather than relying only on broad measures of brain activity.
During behavioral experiments, adjustable tetrodes let investigators relate neuronal firing to sensory processing, learning, memory, or navigation. Because recordings can capture individual neurons and neural populations, the method connects changes in brain activity with behavior. It can also be applied to examine circuit dysfunction, extending its use beyond normal cognitive processing.