Thoracic Drg Imaging
Thoracic Drg Imaging - Related Videos
06:34
In Vivo Thoracic Dorsal Root Ganglia (DRG) Calcium Imaging and ECG Recording for Studying Peripheral Nerve Stimulation
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2024
This study presents a surgical manipulation to expose the T-DRG in anesthetized mice for in vivo calcium imaging, along with synchronous ECG recordings. This method represents a cutting-edge tool for studying peripheral electric nerve stimulation and thoracic visceral organ inputs, as well as their interactions at the primary sensory level.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
FAQs
An optical signal can report different kinds of information depending on what has been labeled or measured. It may show neuronal structure, calcium dynamics, or another physiological response. Interpreting the signal therefore requires attention to the indicator and the experimental condition, rather than treating every brightness change as identical evidence of activity.
Labeled cells can make sensory-neuron structure visible, whereas fluorescent activity indicators are used to track physiological changes such as calcium dynamics. This distinction allows the same imaging approach to address anatomy and function, but the resulting observations answer different questions about thoracic sensory neurons and should be interpreted accordingly.
Changes in optical signals gain meaning when the conditions producing them are specified. Researchers can relate sensory-neuron responses to mechanical or chemical stimuli, or to other controlled experimental circumstances. This linkage helps distinguish an observed physiological response from a structural feature and supports comparisons across investigations of thoracic sensory signaling.
Because thoracic ganglia are associated with sensory pathways from the chest wall and thoracic organs, their signals can be connected to peripheral inputs from those regions. This provides a cellular bridge between local stimuli and sensory-neuron behavior, helping neuroscience studies examine pain, inflammation, and visceral sensation within a defined anatomical context.
A useful workflow identifies the thoracic ganglion and the sensory cells to be observed, applies an appropriate fluorescent label or activity indicator, and records optical responses under defined conditions. The experiment then relates observed structure or signal changes to the tested stimulus or physiological condition, keeping the measurement type and experimental context aligned.
It is particularly useful when a study needs to connect peripheral sensory-neuron behavior with disease-related or stimulus-related changes. Investigators can examine mechanisms associated with pain or inflammation, study visceral sensory responses, and evaluate how thoracic sensory pathways respond under experimental conditions. The resulting observations can also support assessment of potential therapies.