Tissue Culture Procedure
Tissue Culture Procedure - Related Videos
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
FAQs
The culture environment must coordinate nutrient availability with temperature, gas conditions, pH, and substrate attachment. These variables influence whether cells remain viable, multiply, or differentiate. Because each condition can affect biological behavior, controlling them helps researchers distinguish responses caused by an experimental treatment from changes produced by the culture environment itself.
An explant preserves a piece of tissue for study, whereas dissociation separates the tissue into individual cells before placement in culture. This choice changes the organization of the model and the biological behaviors that can be examined. The resulting preparation can therefore emphasize tissue-level responses or more direct analysis of individual cells.
Substrate attachment provides a physical context that can support cell survival, proliferation, or differentiation. In neuronal and glial cultures, this condition also helps maintain cells in a format suitable for observing morphology and cellular responses. Controlling attachment is therefore important when comparing developmental behavior or responses to disease-related conditions.
A typical workflow begins by collecting an explant or dissociating tissue, followed by placing the preparation in sterile nutrient medium. The culture is then maintained under regulated temperature, gas, pH, and substrate conditions. These steps create a controlled system in which survival, proliferation, differentiation, and experimental responses can be examined directly.
Neuroscience researchers can use this approach when they need neuronal or glial models for examining cell development, synaptic signaling, neurotoxicity, or disease-related responses. It also supports testing candidate treatments under controlled conditions. By isolating cellular behavior from the intact organism, the method can help investigate mechanisms that are difficult to study directly in vivo.
Cultured neural tissue can reveal changes in cell survival, proliferation, differentiation, morphology, and responses to experimental conditions. The system is particularly useful for imaging cellular morphology and examining how cells respond to neurotoxic, disease-related, or treatment-related conditions. These observations provide direct cellular evidence for evaluating biological mechanisms and candidate interventions.