Tissue Scarring
Tissue Scarring - Related Videos
07:40
Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
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2022
This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar formation.
05:54
A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring
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Cited by 3 •
2024
This protocol will explain how to establish a hypertrophic scarring murine model that increases mechanotransduction signaling to simulate human-like scarring. This method involves increasing mechanical tension across a healing incision in a mouse and using a specialized device to create reproducible, excessive scar tissue for detailed histological and bioinformatic analyses.
08:20
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
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Cited by 8 •
2020
This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
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Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
07:48
Working with Human Tissues for Translational Cancer Research
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Cited by 4 •
2015
Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.
FAQs
After injury, astrocytes, other glial cells, and immune cells accumulate around the damaged region. Their coordinated response produces extracellular matrix components and helps organize a boundary around the lesion. This cellular arrangement stabilizes the injured area and influences the local environment through interactions among inflammation, tissue structure, and neural connections.
The same response can produce opposing effects. By stabilizing the damaged region and helping maintain a protective boundary, a glial scar may limit the spread of injury. However, that boundary can also restrict axon growth and alter the environment needed for neural reconnection, creating a biological tradeoff between containment and functional recovery.
Extracellular matrix components help reinforce and organize the region surrounding a lesion, but their presence can also make that environment inhibitory to growing axons. Consequently, axons may have difficulty extending through the scar and reconnecting with neural targets. Studying the matrix is therefore important for understanding why structural stabilization may coincide with limited regeneration.
Neuroscience studies examine how scars influence inflammation, neural connectivity, and recovery after brain or spinal cord injury. Researchers also consider whether the scar preserves a protective barrier or contributes to restricted axon growth. These outcomes connect the scar’s cellular and structural features with broader changes in nervous-system function.
A useful approach is to relate scar structure and signaling to two contrasting outcomes: lesion stabilization and restricted repair. Investigators examine how the scar’s organization and cellular signals correspond with inflammatory activity, neural connectivity, and axon growth. This comparison helps identify features worth preserving while revealing effects that may interfere with functional recovery.
The therapeutic goal is not necessarily to eliminate the scar, because its protective barrier can limit additional damage. Instead, research seeks strategies that preserve beneficial stabilization while reducing signals or structural features that inhibit nerve regeneration and functional repair. This approach uses knowledge of scar organization and signaling to balance protection with recovery.