Tmre Co-imaging
Tmre Co-imaging - Related Videos
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
13:05
Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds
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Cited by 12 •
2013
This article shows an optimized procedure for imaging of the neural substrates of auditory stimulation in the songbird brain using functional Magnetic Resonance Imaging (fMRI). It describes the preparation of the sound stimuli, the positioning of the subject and the acquisition and subsequent analysis of the fMRI data.
FAQs
Fluorescence changes provide an optical readout of shifts in the electrochemical gradient across the mitochondrial inner membrane. Because TMRE accumulation depends on mitochondrial polarization, altered signal can indicate that mitochondrial function has changed during neuronal activity, metabolic stress, or injury. Interpreting the fluorescence alongside the second co-imaged signal helps relate mitochondrial responses to specific cellular events.
Pairing TMRE with calcium or voltage indicators connects mitochondrial membrane-potential changes with neuronal signaling in the same experimental context. This allows researchers to examine whether altered mitochondrial function accompanies changes in activity-related signals, rather than observing the mitochondrial response in isolation. The combined measurements therefore provide a more informative view of relationships between neuronal signaling and bioenergetic state.
The co-imaged signal supplies biological context for interpreting mitochondrial changes. Structural or cell-death indicators can show whether altered TMRE fluorescence occurs alongside cellular damage, while activity-related indicators can associate the response with neuronal signaling. Comparing these signals helps investigate whether mitochondrial dysfunction is linked primarily to altered activity, metabolic stress, or injury within living cells or tissues.
The approach begins by observing TMRE fluorescence together with a selected calcium, voltage, structural, or cell-death signal in the same living preparation. Researchers then compare changes in mitochondrial membrane potential with changes in the companion signal. This coordinated observation reveals relationships between mitochondrial behavior and neuronal or cellular events that a single fluorescence measurement would not show.
The appropriate companion indicator depends on the biological question. Calcium or voltage signals are relevant when studying neuronal activity, structural indicators help examine cellular organization, and cell-death indicators support investigations of injury. Combining one of these signals with TMRE enables researchers to examine mitochondrial responses in relation to neuronal signaling, cellular condition, or damage.
Tmre Co-imaging can be used to study how neuronal signaling, metabolic stress, and injury relate to mitochondrial dysfunction. In living cells and tissues, the paired measurements can connect mitochondrial membrane-potential changes with altered bioenergetics and synaptic function. This makes the approach relevant to research on cellular mechanisms associated with neurodegenerative processes.