Ultramicrotome Sectioning
Ultramicrotome Sectioning - Related Videos
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Cross-Sectional Research
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In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
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We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.
03:14
Vibratome Sectioning of Whole Murine Kidney: A Technique to Generate Thin Sections of Murine Kidney
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In this video, we demonstrate the vibratome sectioning of a whole mouse kidney to obtain thin slices. These sliced tissue sections can be stored at low temperatures until further downstream analysis.
08:12
Thin Sectioning of Slice Preparations for Immunohistochemistry
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The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.
15:53
Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning
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Cited by 33 •
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The overall goal of this protocol is to accurately align magnetic resonance imaging (MRI) image volumes with histology sections via the creation of customized 3D-printed brain holders and slicer boxes.
FAQs
The motor-driven feed is central to section quality because it advances the embedded block in controlled increments rather than moving it unpredictably. Each increment determines how much material meets the knife, allowing production of ultrathin slices suitable for high-resolution imaging. This controlled motion helps preserve nanoscale architectural information for subsequent transmission electron microscopy.
Glass and diamond knives serve as the sharp cutting surfaces that the resin-embedded block crosses. The supplied description identifies both as suitable options but does not distinguish their relative performance. Their role is therefore mechanical: they enable controlled separation of ultrathin sections that can be transferred to support grids.
Preserving nanoscale architecture allows investigators to examine relationships among synapses, organelles, myelin, and axons within nervous tissue. These details provide structural evidence for studying neural connectivity and cellular alterations associated with injury or disease. The method therefore supports analysis of fine organization, not merely broad features of the tissue.
A basic workflow begins with a resin-embedded specimen and positions its block against the instrument’s knife. The motor advances the block in controlled increments, producing ultrathin sections as the block passes across the sharp glass or diamond edge. Sections are then collected on support grids, preparing them for transmission electron microscopy and high-resolution examination.
In neuroscience, the technique is useful when investigators need fine structural information about neural tissue. Sections can reveal the organization of synapses, axons, myelin, and organelles, supporting studies of neural connectivity and the cellular effects of injury or disease. Its main contribution is access to preserved architecture at a scale relevant to these questions.
Ultramicrotome sectioning supports neurodegeneration research by making nanoscale structural preservation available for imaging and analysis. Researchers can inspect how cellular architecture is represented in nervous tissue and assess fine features relevant to disease-related changes. The resulting observations provide structural evidence about neural cells and their connections, complementing broader investigations of nervous-system pathology.