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JoVE Journal
Biology

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Thin Sectioning of Slice Preparations for Immunohistochemistry
 

Thin Sectioning of Slice Preparations for Immunohistochemistry

Article DOI: 10.3791/194
April 28th, 2007

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Summary April 28th, 2007

The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.

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