A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:30Mold preparation
- 04:40Sectioning
- 05:47Immunoreaction
The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.
