Oral Glucose Tolerance Test
Oral Glucose Tolerance Test - Related Videos
02:23
Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice
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2023
This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.
08:13
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
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Cited by 151 •
2018
The current article describes the generation and metabolic characterization of high-fat diet-fed mice as a model of diet-induced insulin resistance and obesity. It further features detailed protocols to perform the oral glucose tolerance test and the insulin tolerance test, monitoring whole-body alterations of glucose metabolism in vivo.
06:59
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
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Cited by 7 •
2016
The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.
12:59
Improving Strength, Power, Muscle Aerobic Capacity, and Glucose Tolerance through Short-term Progressive Strength Training Among Elderly People
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Cited by 6 •
2017
The effect of short-term resistance training on elderly people was investigated through the simultaneous use of several methods. Compared to a control group, many improvements were seen, including on muscle aerobic capacity, glucose tolerance, strength, power, and muscle quality (i.e., protein involved in cell signaling and muscle fiber type composition).
02:21
Evaluating the Effect of Continuous Drug Infusion into the Mouse Brain on Peripheral Glucose Levels
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2025
This video demonstrates the effect of continuous drug infusion into the mouse brain on blood glucose levels. Glucose metabolism is assessed by collecting blood from a fasted mouse's tail and measuring glucose levels before and after oral glucose administration. Insulin tolerance is evaluated by injecting insulin in the mouse, with glucose levels recorded at specific intervals.
FAQs
The glucose curve captures both the initial response to the oral load and the subsequent return toward baseline. A pronounced rise may reflect glucose entering the circulation faster than it is being handled, whereas delayed clearance can indicate inadequate insulin action, reduced tissue glucose uptake, or impaired metabolic regulation. Examining the full time course provides more information than relying on a single measurement.
Insulin secretion increases after glucose enters the circulation and signals tissues to remove glucose from the blood. The observed response therefore depends on both pancreatic activity and how effectively tissues respond to insulin. A weaker secretory response or reduced tissue sensitivity can alter the magnitude and duration of hyperglycemia, helping investigators distinguish different contributors to abnormal glucose regulation.
Changes in the test curve can indicate where a drug acts within glucose regulation. An altered early rise may suggest an effect on glucose absorption, while improved clearance may reflect enhanced insulin sensitivity, secretion, or tissue uptake. Comparing responses under controlled conditions allows pharmacologists to evaluate glycemic effects without assuming that every change results from the same mechanism.
The procedure begins with fasting, followed by administration of a standardized glucose solution. Blood samples are then collected at defined intervals to track glucose changes over time. Consistent preparation, glucose dosing, sampling timing, and interpretation are important because variation in these conditions can change the measured curve and make comparisons between individuals or treatment conditions less reliable.
Researchers use the test when they need to characterize how a compound influences glucose regulation after a controlled challenge. It can support evaluation of effects on insulin sensitivity, glucose absorption, tissue glucose uptake, or overall glycemic control. Repeated measurements across the response period help reveal treatment-related changes that may not be evident from a single glucose value.
The collected measurements can reveal impaired glucose tolerance and patterns consistent with diabetes-related metabolic dysfunction. In research, the results also provide a functional readout for comparing glucose regulation across treatment conditions or metabolic states. Interpretation should consider the complete response pattern, because the timing and extent of glucose rise and clearance both contribute to the overall finding.