Name: how cellular respiration makes atp
Uploaded: 2019-08-01T09:24:07.000Z
Duration: 1 min 31 s
Channel: JoVE

K12 Standards

Virginia  Standards of Learning

HIgh School

Life Science

LS.4

LS.4.b

ATP Yield

- [Anlatan] Hücresel solunumda 30-32 ATP molekül getirisi kademeli üretimle elde edilir.
 Bu üretim sürecine yüksek enerji molekülleri, glikolizde bulunan iki ATP ve iki NADH, pirüvat oksidasyonundaki iki NADH, iki ATP, altı NADH, sitrik asit döngüsündeki iki FADH2 ve oksidatif fosforilasyonda görülen yaklaşık 26 ya da 28 ATP dahildir.
 Bu ATP miktarının üç sebebi vardır.
Birincisi, elektron taşıyıcısı olan NADH ve FADH2'nin hidrojen iyonlarının iç membrana pompalanmasına sebep olarak dolaylı yoldan ATP salınımına yol açmalarıdır.
 Bu salınım oldukça düşük miktardadır.
 Bu değer 2.5 ile 1.5 ATP aralığındadır.
İkincisi, glikolizle oluşan NADH'nin mitokondriyal membrandan geçemediği için yüksek enerji elektronlarını mitokondriyadaki diğer elektron taşıyıcılarına iletme zorunluluğudur.
 Buna ek olarak hücre türüne göre her biri 1.5 ya da 2.5 ATP değerinde FADH2 ya da NADH üretmeleridir.
 Üçüncüsü, solunumla elde edilen enerji aynı zamanda pirüvatın mitokondriyal membrana transferi gibi diğer aktiviteler için de kullanılmasıdır.
 Bu süreç 30 ya da 32 ATP değerindedir.

atp verimi

Hücresel solunum, glikoz molekülü başına 30-32 ATP molekülü üretir. ATP'nin çoğu oksidatif fosforilasyondan ve elektron taşıma zincirinden (ETC) kaynaklansa da önceden 4 ATP kazanılır (2'si glikolizden ve 2'si sitrik asit döngüsünden).

ETC, iç mitokondriyal membrana gömülüdür ve dört ana protein kompleksi ve bir ATP sentaz içerir. NADH ve FADH2, elektronları bu komplekslere geçirir ve bu da protonları zarlar arası boşluğa pompalar. Protonların bu dağılımı, zar boyunca bir konsantrasyon gradyanı oluşturur. Gradyan, protonlar ATP sentaz yoluyla mitokondriyal matrise geri aktığında ATP üretimini yönlendirir.

NADH'nin kompleks I, kompleks I ve III'e geçtiği her 2 giriş elektronu için her biri 4 proton pompalar ve kompleks IV, toplam 10 proton olmak üzere 2 proton pompalar. Kompleks II, NADH tarafından başlatılan elektron zincirine dahil değildir. Bununla birlikte FADH 2 , 2 elektronu kompleks II'ye geçirir, bu nedenle FADH 2 başına toplam 6 proton pompalanır; kompleks III yoluyla 4 proton ve kompleks IV yoluyla 2 proton.

1 ATP'yi sentezlemek için dört protona ihtiyaç vardır. Her NADH için 10 proton pompalandığından, 1 NADH 2,5 (10/4) ATP verir. Her FADH 2 için altı proton pompalanır, bu nedenle 1 FADH 2 1.5 (6/4) ATP verir.

Hücresel solunum, glikoz molekülü başına maksimum 10 NADH ve 2 FADH 2 üretir. Tek bir NADH 2.5 ATP ürettiğinden ve tek bir FADH 2 1.5 ATP ürettiğinden, oksidatif fosforilasyon ile 25 ATP + 3 ATP üretilir. Oksidatif fosforilasyondan önce, glikoz molekülü başına maksimum 32 ATP veren dört ATP üretilir.

Önemli olarak, glikoliz sitozolde meydana gelir ve ETC mitokondride (ökaryotlarda) bulunur. Mitokondriyal membran, NADH'ye geçirgen değildir, bu nedenle glikoliz tarafından üretilen 2 NADH'nin elektronlarının mitokondriye gönderilmesi gerekir. Mitokondriye girdikten sonra, elektronlar NAD + veya FAD'ye geçirilebilir. Elektron taşıyıcısına bağlı olarak farklı ATP verimleri göz önüne alındığında, toplam hücresel solunum verimi glikoz molekülü başına 30 ila 32 ATP'dir.

ATP Verimi

Hücresel solunum, glikoz molekülü başına 30 - 32 ATP üretir. ATP'nin çoğu oksidatif fosforilasyon ve elektron taşınmasından kaynaklansa da ...

Education

JoVE Core

Biology

Biyoloji

Cellular Processes

Hücresel Süreçler

Cellular Respiration

Hücresel Solunum

KEY TERMS AND CONCEPTS

TEMEL TERİMLER VE KAVRAMLAR

how cellular respiration makes atp

Cellular respiration makes ATP from glucose in several stages. The process usually produces 30 to 32 ATP per glucose molecule. Most of that ATP comes from oxidative phosphorylation and the electron transport chain, while 4 ATP are made earlier, with 2 from glycolysis and 2 from the citric acid cycle.

The electron transport chain, or ETC, sits in the inner mitochondrial membrane. It has four main protein complexes and ATP synthase, the enzyme that makes ATP. NADH and FADH2 deliver electrons to these complexes. As electrons move through the chain, the complexes pump protons into the intermembrane space and build a gradient across the membrane.

That proton gradient powers ATP production. Protons flow back into the mitochondrial matrix through ATP synthase, and this flow drives ATP formation. For every 2 electrons that NADH sends through complex I, 10 protons are pumped in total. Complex I and complex III each pump 4 protons, and complex IV pumps 2 protons. Complex II is not part of the electron chain started by NADH.

FADH2 follows a different path. It passes 2 electrons to complex II, so a total of 6 protons are pumped per FADH2. In this case, complex III pumps 4 protons and complex IV pumps 2 protons. Four protons are needed to make 1 ATP. That means 1 NADH yields 2.5 ATP, and 1 FADH2 yields 1.5 ATP.

Per glucose molecule, cellular respiration can produce up to 10 NADH and 2 FADH2. Since each NADH makes 2.5 ATP and each FADH2 makes 1.5 ATP, oxidative phosphorylation produces 25 ATP from NADH and 3 ATP from FADH2. Adding the 4 ATP made before that stage gives a maximum of 32 ATP per glucose molecule. In eukaryotes, glycolysis happens in the cytosol, and the ETC is in the mitochondria.

The mitochondrial membrane does not let NADH cross. Because of that, the electrons from the 2 NADH made in glycolysis must be shuttled into the mitochondria. Once inside, those electrons may be passed to NAD+ or FAD. Since the shuttle path can change the carrier used, the total ATP yield of cellular respiration is usually 30 to 32 ATP per glucose molecule.

How Cellular Respiration Makes ATP

what is glycolysis?

Glycolysis: How Cells Break Down Glucose

Glycolysis is the first stage of cellular respiration and a key way cells break down glucose to make energy. Cellular respiration converts food energy from macromolecules into ATP, the cell’s main energy currency. Glycolysis also creates intermediate molecules that can feed into other pathways. In this pathway, one six-carbon sugar such as glucose is split into two three-carbon molecules, including pyruvate. The process produces a net gain of two ATP molecules, since four ATP are made and two...

energy-requiring steps of glycolysis

Glycolysis: Glucose Trapping and Splitting

Glycolysis begins in the cytosol, where glucose is prepared for use as energy. This process has two phases: an energy-requiring phase and an energy-releasing phase. The first phase changes glucose into a more reactive, unstable form before it can move into later steps. In the first step, glucose is phosphorylated, which means a phosphate group is added from ATP. This produces glucose 6-phosphate. The added phosphate is negatively charged, so it cannot cross the hydrophobic cell membrane. As a...

energy-releasing steps of glycolysis

Glycolysis: ATP and NADH Formation

Glycolysis produces ATP and NADH during its energy-releasing phase. These reactions are the last steps of glycolysis and convert glyceraldehyde-3-phosphate, or G3P, into pyruvate. The phase is often described as steps 6 to 10, and it happens twice because two 3-carbon sugars are made earlier in glycolysis. The sixth step begins with two linked events. G3P is oxidized, which means it loses hydrogen, and it is phosphorylated, which means a phosphate group is added. NAD+ acts as the electron...

pyruvate oxidation

How Pyruvate Becomes Acetyl CoA

Pyruvate is prepared for the next stage of cellular respiration inside the mitochondria. After glycolysis, the charged pyruvate molecules enter the mitochondria by active transport. There, they go through three enzymatic reactions so the energy stored in pyruvate can be used by the cell. First, pyruvate dehydrogenase removes the carboxyl group from pyruvate. This step releases carbon dioxide. Next, the stripped molecule is oxidized, and the electrons are picked up by NAD+ to make NADH. The...

the citric acid cycle

Citric Acid Cycle Energy and Products

The citric acid cycle is a key energy-making pathway in cells. It is also called the Krebs cycle or the TCA cycle. In eukaryotic cells, it takes place in the matrix of mitochondria. In prokaryotic cells, it occurs in the cytoplasm. The cycle starts with acetyl CoA, which enters the pathway after pyruvate oxidation. That earlier step makes two acetyl CoA molecules per glucose molecule. For that reason, the citric acid cycle runs twice for each glucose molecule. The pathway includes eight...

electron transport chains

ATP Production by the Electron Transport Chain

ATP production in the electron transport chain is the final stage of cellular respiration. This stage is part of oxidative phosphorylation, which has two steps: the electron transport chain and chemiosmosis. In eukaryotic cells, the electron transport chain sits in the inner mitochondrial membrane. Its main job is to build a proton gradient. That gradient is then used during chemiosmosis to make ATP. The process also regenerates the electron carriers NAD+ and FAD, which are needed again in...

chemiosmosis

ATP Production by Chemiosmosis

ATP production by chemiosmosis is part of oxidative phosphorylation, the efficient stage of cellular respiration that makes most of the cell’s ATP. Oxidative phosphorylation includes two linked processes: the electron transport chain and chemiosmosis. Together, they help generate adenosine triphosphate, or ATP, the main energy molecule used by cells. The electron transport chain is a series of protein complexes in the inner mitochondrial membrane. These complexes pass electrons through a set...

electron carriers

Electron Carriers in Cellular Respiration

Electron carriers help move electrons through cellular respiration. They act like shuttles that can accept electrons, which means they are reduced, or give electrons away, which means they are oxidized. This changing between oxidized and reduced forms is essential for energy production. As glucose breaks down into carbon dioxide and water, it loses electrons at several stages. Electron carriers pick up those electrons and store them temporarily. They then release the electrons into the...

fermentation

Fermentation Pathways Without Oxygen

Fermentation is a metabolic pathway that lets cells make energy without oxygen. It begins after glucose is split into two pyruvate molecules during glycolysis. Glycolysis is the same first step used in aerobic respiration. When oxygen is unavailable, pyruvate does not enter aerobic respiration. Instead, cells convert pyruvate into other organic molecules through pathways that depend on the organism. A key purpose of fermentation is to regenerate NAD+ from NADH so glycolysis can keep running. <br...

dietary connections

How Food Becomes ATP

Food becomes ATP through connected steps in cellular respiration. Carbohydrates, proteins, and lipids all feed into these pathways. The body can use foods such as apples, cheese, and guacamole as sources of energy. Carbohydrate digestion starts with enzymes that break complex carbohydrates into simple sugars. Glucose can enter glycolysis directly. Fructose and galactose must first be converted into sugars that are intermediates of the glycolytic pathway. Proteins are digested into amino acids.

introduction to cellular respiration

Cellular Respiration: ATP Production Steps

Cellular respiration is the process cells use to turn energy from food into ATP. ATP, or adenosine triphosphate, stores energy in chemical bonds that can be released quickly when cells need it. Much of the energy released during respiration becomes heat, but some of it is captured to make ATP. This process depends on oxidation-reduction, or redox, reactions. In these reactions, oxidation means electron loss and reduction means electron gain. The electron carriers NAD+ and FAD, along with their...

products of the citric acid cycle

Citric Acid Cycle Output in Cellular Respiration

The citric acid cycle is a key stage of aerobic respiration in cells. It is also called the Krebs cycle or the tricarboxylic acid (TCA) cycle. Plants and animals use this oxygen-requiring pathway as part of cellular respiration. This cycle happens after glycolysis and pyruvate oxidation. Glycolysis makes two pyruvate molecules from each glucose molecule. During pyruvate oxidation, each pyruvate becomes acetyl-CoA, which is the input for the citric acid cycle. That means one glucose molecule...

outcomes of glycolysis

Glycolysis Products in Cellular Respiration

Glycolysis is the first step of cellular respiration and breaks glucose into smaller molecules. Cells use this process to pull energy from glucose over a series of chemical reactions. Cellular respiration can happen with oxygen or without oxygen. In both aerobic and anaerobic cellular respiration, glycolysis makes a net gain of two pyruvate molecules, two NADH molecules, and two ATP molecules. Four ATP molecules are produced, but two ATP molecules are used earlier in the pathway. Glycolysis...

atp yield

Cellular respiration makes ATP from glucose in several stages. The process usually produces 30 to 32 ATP per glucose molecule. Most of that ATP comes from oxidative phosphorylation and the electron transport chain, while 4 ATP are made earlier, with 2 from glycolysis and 2 from the citric acid cycle. The electron transport chain, or ETC, sits in the inner mitochondrial membrane. It has four main protein complexes and ATP synthase, the enzyme that makes ATP. NADH and FADH2 deliver electrons to...

key terms and concepts

See how cellular respiration turns glucose into ATP through glycolysis, the citric acid cycle, and the electron transport chain, for a total of 30 to 32 ATP.

How Cellular Respiration Makes ATP | JoVE K12

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JoVE Core serisi, biyolojinin ana konseptlerini açıklayan 300 adet kısa ve kolay anlaşılabilir olan video dersleriyle ve günümüz laboratuvarlarında yürütülen gerçek araştırmaları gösteren 150'den fazla \"bilim insanları iş başında\" videolarıyla biyolojiye hayat vermektedir.

Next Generation Science Standards

High School

ecosystems-interactions-energy-and-dynamics

Ecosystems: Interactions, Energy, and Dynamics

HS-LS2-5

Florida Department of Education

SC.912.L.18

SC.912.L.18.5

Texas Essential Knowledge and Skills

BIO.11

BIO.11.A

new-york-state-science-learning-standards

New York State Science Learning Standards

matter-and-energy-in-organisms-and-ecosystems

Matter and Energy in Organisms and Ecosystems

HS-LS1-7.

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

HS-LS1-7

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How Cellular Respiration Makes ATP

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