12.16
Nükleer Olmayan Kalıtım
Çoğu DNA, bir hücrenin çekirdeğinde bulunur. Bununla birlikte, hücre sitoplazmasındaki bazı organeller — kloroplastlar ve mitokondri gibi— ayrıca ke…
- [Anlatan] Ökaryotik hücrelerin içinde, mitokondriler ve kloroplast içeren bir bitki hücresi organel kategorisi olan plastidler, sitoplazmada bağımsız olarak çoğalırlar ve her birinin, kendi çoğalmasını ve işlevini yöneten gen setleri vardır. Mitokondriyal ve plastid DNA'sı, küçük, dairesel DNA molekülleri şeklindedir ve bunlara çekirdeğe özgü olmayan veya sitoplasmik DNA denir. Her organelde, DNA'sının birçok eş kopyası bulunur, bunun tersine çekirdekte ise her kromozomun tipik olarak sadece iki kopyası bulunur.
Ayrıca, hücre bölünmesi sırasında, organeller yeni yavru hücrelere rastgele ayrılırlar, böylece aktarılan, çekirdeğe özgü olmayan DNA belli bir patern izlemez. Hayvanlardaki ve çoğu bitkideki çoğalma sırasında, zigottaki sitoplazma yumurtadan gelir. Bu yüzden, çekirdek DNA'sına iki ebeveyn de eşit katkıda bulunurken, çekirdeğe özgü olmayan DNA sadece anneden aktarılır.
Bu, mitokondrilerdeki mutasyonların sonucu olan metabolik rahatsızlıklar gibi belli özelliklerin, anne soyunda izlenebileceği anlamına gelir. Ayrıca bitkilerdeki, yaprak rengi gibi bazı fiziksel özellikleri, çekirdeğe özgü olmayan genler kontrol eder ve bunlar da tek ebeveyn tarafından aktarılır.
View the full transcript and gain access to JoVE Core videos
Q1: What is non-nuclear inheritance and how does it differ from nuclear inheritance?
Non-nuclear inheritance describes the inheritance of genes from organelles like mitochondria and chloroplasts rather than the nucleus. Unlike nuclear DNA, which follows Mendelian patterns with contributions from both parents, non-nuclear DNA is inherited maternally because sperm cells do not contribute organellar DNA to zygotes. Organelles carry many identical copies of their DNA, whereas the nucleus typically contains only two copies of each chromosome.
Q2: Why do mitochondria and chloroplasts contain their own DNA?
The endosymbiotic theory explains that mitochondria and chloroplasts were once independent prokaryotes that entered a symbiotic relationship with host eukaryotic cells. This mutually beneficial partnership allowed these organelles to retain their own DNA while functioning within the cell. Mitochondria break down glucose for energy through oxidative phosphorylation, while chloroplasts conduct photosynthesis in plant cells.
Q3: How is maternal inheritance different from typical Mendelian inheritance patterns?
Maternal inheritance occurs because the egg cell contributes its organelles to the zygote, while sperm cells do not. This means non-nuclear DNA passes exclusively from mother to offspring, unlike nuclear chromosomes inherited from both parents. Traits controlled by non-nuclear genes, such as certain leaf colors in plants or mitochondrial metabolic disorders, follow matrilineal inheritance patterns rather than Mendelian ratios.
Q4: What happens to organellar DNA during cell division?
During cell division, mitochondria and chloroplasts are randomly segregated into daughter cells, so non-nuclear DNA does not follow a predictable inheritance pattern like nuclear DNA. Each organelle carries many identical copies of its DNA, but the distribution of these copies to daughter cells is random. This random segregation contributes to variable expression of traits controlled by non-nuclear genes.
Q5: How many genes do mitochondria and chloroplasts contain?
Mitochondrial DNA consists of approximately 37 genes, many contributing to oxidative phosphorylation, the process that generates cellular energy. Chloroplast DNA contains about 100 genes, with many involved in photosynthesis. These organellar genomes are much smaller than the nuclear genome, reflecting their specialized roles in energy production and photosynthetic processes.
Q6: What is the structure of mitochondrial and chloroplast DNA?
Mitochondrial and chloroplast DNA exists as small, circular DNA molecules, similar to prokaryotic DNA. This circular structure reflects their evolutionary origin as independent organisms. Each organelle carries many identical copies of its circular DNA, contrasting sharply with the nucleus, which typically contains only two copies of each linear chromosome.
Q7: Can mitochondrial mutations cause inherited diseases?
Yes, mutations in mitochondrial DNA can cause metabolic disorders that are inherited maternally. Because mitochondria generate cellular energy through oxidative phosphorylation, mutations affecting this process can disrupt energy production and cause disease. These mitochondrial disorders can be traced through pedigree analysis and disease inheritance patterns, as all offspring inherit their mitochondria exclusively from the mother.