Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Özet

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Özet

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protokol

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development için bir düzeltme (erratum) yayınlanmıştır.

Burada ve Caralt'ta kullanılan insan RCTEC/RCTE hücre stokları ve ark., 2015, Uzarski ve ark., 2015 yılındaki örnekler, başlangıçta 2001 yılında bir MTA aracılığıyla, o dönemde St. Louis Üniversitesi'nde olan Dr. Loghman-Adham tarafından ortak yazar Dr. Wandinger-Ness'e (New Mexico Üniversitesi) sağlanmıştır. SV40 ile ölümsüzleştirilmiş insan RCTEC/RCTE hücreleri, şu şekilde detaylandırıldığı üzere distal tübül hücre hattı olarak karakterize edilmiştir: Loghman-Adham ve ark., 2003 yılında, RCTEC/RCTE hücre stoklarının en erken pasajlarına uygulanan güncel kısa tandem tekrar (STR) DNA dizileme (IDEXX BioResearch tarafından gerçekleştirilen) sonucunda, hücrelerin karma soyda olduğunun ortaya çıktığı görülmüştür. QIAxcel kapiler elektroforez kullanılarak PCR ürünleri üzerinde yapılan ileri analizler, köpek kaynaklı bir ürünün varlığını göstermiştir. Ürün dizilenmiş ve köpek kökenli olduğu saptanmıştır. Köpeğe özgü bir dizi STR markörü ile numune karşılaştırılmış ve numunenin MDCK hücre hattı ile %92 benzerliğe sahip bir genetik profile sahip olduğu görülmüştür.  Bu yayında kullanılan sonraki hücre stoklarının STR profillemesi yapılmış ve %100 MDCK soyuna kayma olduğu görülmüştür. Daha önce sanıldığı gibi insan değil, köpek kökenli olmasına rağmen MDCK de benzer şekilde bir distal tübül epitelyal hücre hattıdır. Bu nedenle, burada ve şurada bu hücreler kullanılarak yapılan önceki yorumlar ve çıkarılan sonuçlar... Caraltı ve ark., 2015, Uzarski ve ark., 2015 yılına ait veriler hala geçerliliğini korumaktadır; ancak titizlik, tekrarlanabilirlik ve diğer araştırmacılar tarafından deneysel doğrulama sağlanması amacıyla bu yanlış tanımlamayı bildiriyoruz. Bu bilgi ayrıca Uluslararası Hücre Hattı Kimlik Doğrulama Komitesi (ICLAC) veri tabanına (http://iclac.org) kaydedilmek üzere raporlanacaktır.