Bioreactor Culture
Bioreactor Culture - Related Videos
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
12:39
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
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Cited by 7 •
2008
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
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Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
FAQs
Sensors track conditions such as temperature, pH, dissolved oxygen, nutrient supply, and mixing, while control systems adjust the culture environment when those parameters change. This coordinated regulation helps keep cells or microorganisms within conditions that support biological production. Consistent control also makes it easier to compare experiments, evaluate cell performance, and maintain reproducible manufacturing outcomes.
Agitation distributes nutrients and other resources throughout the vessel, while aeration supports the transfer of gases into the culture. Together, these processes improve mass transfer and reduce uneven conditions within the growing population. Their effectiveness influences how consistently cells, microorganisms, or tissues encounter the regulated environment, which can affect product formation and overall culture performance.
Temperature, pH, dissolved oxygen, nutrient availability, and mixing are central variables because they shape the environment experienced by the cultured material. Changes in any of these conditions can influence cell performance, microbial growth, tissue development, or product yield. Monitoring them provides a basis for identifying process changes and maintaining consistency as the culture progresses.
A typical workflow begins by establishing the desired culture conditions, then using sensors to monitor temperature, pH, dissolved oxygen, nutrient supply, and mixing during growth. Control systems help maintain those parameters, while researchers assess cell performance and product yield. Recording these observations supports process evaluation and helps determine whether the culture remains consistent over time.
Bioengineers use this approach when biological material must be grown under controlled conditions or when production needs to move beyond a small laboratory experiment. Applications described for bioreactor culture include vaccines, therapeutic proteins, biofuels, and engineered tissues. The same controlled environment supports both research studies and the development of processes intended for industrial manufacturing.
Bioreactor culture provides a pathway for extending biological processes from laboratory experiments toward industrial manufacturing while continuing to monitor important conditions. Researchers can evaluate cell performance, product yield, and process consistency as the process grows in scale. These measurements help reveal whether the biological system continues to perform reliably under the larger production framework.