Drug Permeation
Drug Permeation - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
05:54
Controlled Odor Mimic Permeation Systems for Olfactory Training and Field Testing
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Cited by 1 •
2021
The Controlled Odor Mimic Permeation System is a simple, field-portable, low-cost method of odor delivery for olfactory testing and training. It is constructed of an odorant retained on an adsorbent material and contained inside of a permeable polymer bag allowing controlled release of the odorant vapor over time.
02:29
Modeling Drug-Induced Hemorrhage in Zebrafish Larva
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2025
In this video, fertilized zebrafish embryos are dechorionated under a stereo microscope and then exposed to a cholesterol synthesis inhibitor, leading to the development of blood vessel ruptures and brain hemorrhages in the larvae. Hemorrhagic larvae, identifiable by distinct red spots, are subsequently separated under a microscope for further analysis.
05:10
Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System
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Cited by 4 •
2016
Here we describe a protocol using the web-based drug repurposing hypothesis generation tool: "RE:fine Drugs." This protocol can be modified to a user's preferences at the level of the query type (gene, drug or disease) and/or the range of available advanced options.
FAQs
A compound may cross a biological barrier by passive diffusion, by using membrane proteins, or by passing between adjacent cells. Passive diffusion follows a concentration gradient, whereas protein-mediated transport depends on membrane components. Passage between cells reflects the barrier’s structure. Comparing these routes helps bioengineers select delivery strategies suited to a particular tissue.
Molecular size, polarity, and lipid solubility shape how readily a therapeutic compound crosses a barrier. Barrier structure and local conditions also modify the outcome, so the same compound may behave differently in skin, intestinal epithelium, or the blood-brain barrier. Evaluating these variables helps explain differences in absorption, distribution, and efficacy.
Barrier structure determines whether a compound encounters a route through cells, between cells, or across membrane components. Differences among skin, intestinal epithelium, and the blood-brain barrier therefore influence transport even when the therapeutic compound is unchanged. Accounting for structure is essential when designing models intended to predict behavior in the body.
Researchers use permeation studies to examine whether a delivery system, formulation, or biomaterial supports movement across a selected biological barrier. The resulting transport behavior can guide optimization rather than relying only on the compound itself. In bioengineering, this approach helps connect material design with the desired absorption, distribution, or targeted delivery outcome.
In vitro barrier models provide a controlled way to investigate transport before considering behavior in the body. Bioengineers use them to study how barrier characteristics and delivery designs influence compound movement. Their central value is predictive: well-designed models can support formulation development and help identify approaches that may improve efficacy or safety.
Permeation research can support delivery through skin, across intestinal epithelium, or toward the blood-brain barrier. These studies inform strategies for improving absorption, controlling distribution, and directing therapies toward selected sites. By linking barrier transport with formulation and biomaterial design, bioengineering approaches can contribute to safer and more effective treatments.