High-throughput Polymer Screening
High-throughput Polymer Screening - Related Videos
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
02:49
High-Throughput Identification of Bacteria-Repellent Polymers via Microarray Screening
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2025
Source: Venkateswaran, S., et al. High-throughput Identification of Bacteria Repellent Polymers for Medical Devices. J. Vis. Exp. (2016)This video demonstrates a high-throughput method to identify bacteria-repellent polymers using a microarray slide exposed to mixed bacterial cultures. Bacterial binding is assessed by DAPI fluorescence, where reduced signal indicates polymers with minimal surface attachment.
13:37
Polymer Microarrays for High Throughput Discovery of Biomaterials
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Cited by 32 •
2012
A description of the formation of a polymer microarray using an on-chip photopolymerization technique. The high throughput surface characterization using atomic force microscopy, water contact angle measurements, X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry and a cell attachment assay is also described.
03:49
Chromogenic Polymer Hydrogel-Based Enzyme Screening Assay: A High Throughput Method to Screen the Carbohydrate-Active Enzymes Using Synthetic Substrates
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2025
In this video, we demonstrate a high throughput assay for carbohydrate-active enzyme screening using insoluble chromogenic polymer hydrogel substrates. The enzyme degrades these substrates into a soluble colored product that can be quantified by measuring the color intensity of the product.
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Cited by 7 •
2016
This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
FAQs
The approach changes polymer composition, molecular structure, or processing conditions across a library, then compares the resulting chemical, physical, or biological performance. Because many related materials are evaluated in parallel, researchers can connect specific structural or processing changes with differences in function and identify combinations that merit further development.
Researchers can vary the polymer’s composition, molecular structure, and processing conditions. These variables may alter chemical behavior, physical performance, or biological responses, allowing the library to represent many candidate materials rather than a single formulation. Systematically changing these features helps identify which material characteristics are most relevant to the desired bioengineering outcome.
Parallel or combinatorial synthesis paired with automated testing allows many polymer samples to be produced and assessed within the same screening effort. This increases the number of material comparisons while reducing the time and resources required for conventional one-at-a-time experiments. The resulting comparisons can accelerate discovery by narrowing a large library to more promising candidates.
Screening can compare chemical, physical, and biological properties, with the relevant measurements depending on the intended use. In bioengineering, important criteria include biocompatibility, degradation, mechanical strength, and cell interaction. Considering these properties together helps researchers distinguish materials that perform well in one category from candidates that provide a more suitable overall profile.
A typical workflow begins by creating a polymer library through parallel or combinatorial synthesis. Researchers then apply automated testing to measure selected chemical, physical, or biological properties across the samples. Comparing those results links material structure or processing conditions to performance and supports selection of polymers for more focused investigation or application development.
The strategy is useful when researchers must compare many candidate materials for applications such as drug delivery, tissue engineering, biosensors, or medical devices. Each area may require a different balance of biocompatibility, degradation, mechanical strength, and cell interaction. Screening helps evaluate these alternatives systematically and supports faster identification of polymers suited to the intended function.