Host Cell Transcription
Host Cell Transcription - Related Videos
03:35
Analysis of Virus-Induced Host Transcriptional Suppression Using Flow Cytometry
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2026
Source: Kalveram, B., et al. Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis. J. Vis. Exp. (2013)This video demonstrates the use of click chemistry and flow cytometry to measure virus-induced suppression of host-cell transcription. Mammalian cells are infected with a virus that expresses a nuclear virulence protein disrupting the host transcription factor complex, while control cells remain transcriptionally active. Incorporation of 5-ethynyl uridine...
04:15
Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System
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2025
This video demonstrates a permeable membrane insert system to investigate the impact of bacterial toxins on host cells in a controlled and isolated manner. This approach allows for the selective exposure of the cells to the toxins while maintaining a physical separation that mimics the natural host-pathogen interaction.
04:29
Visualizing Transcriptional Suppression in Virus-Infected Cells
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2026
Source: Kalveram, B. et al. Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis. J. Vis. Exp. (2013)This video demonstrates the use of click chemistry and fluorescence microscopy to visualize nascent RNA transcription in mammalian cells. It highlights how viral infection suppresses host transcription by comparing fluorescent signals between infected and control cells.
09:35
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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Cited by 15 •
2013
This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.
02:36
Bacteriophage Synthesis Using a Cell-Free Transcription-Translation System
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2026
Source: Rustad, M., et al. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System. J. Vis. Exp. (2017).This video demonstrates the synthesis of bacteriophages using a cell-free transcription–translation (TXTL) system. A master mix is prepared using a bacterial crude extract, an energy mix, an amino acid mix, an inhibitor for a DNA-degrading enzyme, and bacteriophage DNA, then incubated. The in vitro molecular machinery enables viral protein expression and DNA...
FAQs
Transcription factors recognize and bind regulatory DNA sequences, helping recruit RNA polymerase to a promoter. The polymerase then unwinds the DNA template and assembles an RNA strand complementary to that template. This sequence of molecular events connects regulatory information in DNA with production of an RNA molecule, allowing changes in regulatory control to influence gene expression and cellular function.
A promoter provides the defined DNA context where RNA polymerase begins RNA synthesis, while transcription factors help determine whether the polymerase is recruited there. Regulatory DNA sequences therefore act as control points rather than passive templates. Their activity can alter expression patterns associated with development, metabolism, and responses to environmental signals, making promoter regulation central to cellular adaptation.
In eukaryotic cells, the initial transcript is not necessarily the final messenger RNA. The pre-mRNA undergoes capping, splicing, and polyadenylation before serving as the processed product of gene expression. These steps expand the regulatory sequence between transcription and cellular function, so studies of RNA production must distinguish the initial transcript from its processed form.
Pathogens can redirect host transcriptional machinery, changing how the cell uses its own regulatory system. This makes host cell transcription relevant to infection biology: researchers can examine how regulatory DNA, transcription factors, and RNA polymerase are affected when a pathogen alters host processes. The same framework links molecular interference to broader changes in host cellular function.
A study of host cell transcription can follow a sequence from regulatory DNA and transcription-factor binding, through RNA polymerase recruitment and RNA synthesis, to pre-mRNA processing. Researchers can then relate these molecular stages to gene-expression patterns and cellular outcomes such as development, metabolism, or environmental responses. This workflow helps connect molecular events with biological function.
Researchers study this process to explain how cells control development, metabolism, and responses to environmental signals, and to investigate how pathogens redirect host machinery. The topic also supports efforts to engineer cells for therapeutic and biotechnology applications. These uses make transcription relevant both to fundamental biology and to applied research involving controlled changes in cellular function.