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JoVE Journal
Immunology and Infection

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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
 

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Article DOI: 10.3791/50809
August 9th, 2013

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Summary August 9th, 2013

This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.

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