Sterile Saline
Sterile Saline - Related Videos
12:29
Sterile Tissue Harvest
0 Views •
2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
03:15
A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats
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2025
This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.
01:30
Cleaning, Sterilization, and Disinfection
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2023
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease. Cleaning The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:17
Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water
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2025
Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...
06:29
Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
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Cited by 2 •
2016
Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
FAQs
Its approximately 0.9% sodium chloride concentration helps maintain osmotic conditions similar to body fluids. This reduces the net movement of water across cell membranes, lowering the likelihood that cells will swell from water entry or shrink from water loss. Consequently, biological material can be rinsed, diluted, or handled with less osmotic disruption.
Sterility helps prevent microorganisms present in the solution from being introduced into cells, tissues, specimens, or laboratory preparations. This supports procedures that require a clean fluid and reduces an avoidable source of biological contamination. The benefit concerns microbial cleanliness, while the salt concentration separately supports compatibility with the osmotic conditions surrounding cells.
The absence of nutrients and added growth factors makes the solution suitable for washing or suspending biological material without intentionally encouraging microbial growth. It also helps avoid adding growth-promoting components that could alter an experimental preparation. This makes saline useful when the goal is handling or transfer rather than cultivating cells or microorganisms.
Salt concentration affects the balance of water movement across cell membranes. A formulation near the osmotic conditions of body fluids helps limit pronounced volume changes, whereas an unsuitable balance could contribute to cellular swelling or shrinking. In biology, this property is important when preparing, rinsing, diluting, or examining cells and tissues.
During preparation, the solution can rinse or suspend biological material while providing a physiologically compatible fluid environment. Its sterile, nutrient-free composition supports handling without intentionally promoting microbial growth or adding growth factors. These properties make it relevant to laboratory workflows in which cells or tissues must be prepared before further examination or processing.
Researchers may select it when a specimen needs contact with a clean fluid that limits osmotic disruption during collection or examination. In microscopy and related laboratory procedures, it can help rinse or suspend biological material without introducing nutrients intended to promote growth. Its value therefore combines cleanliness, physiological compatibility, and minimal alteration of the preparation.