Two-photon Micropuncture
Two-photon Micropuncture - Related Videos
07:37
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
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Cited by 5 •
2018
We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.
06:43
Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture
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2023
This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.
04:32
Two-Photon In Vivo Imaging of the Mouse Retina
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2025
Source: Wang, Z., et al. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (2021).This video demonstrates a technique for in vivo retinal imaging using two-photon microscopy. Anesthetized mice are prepared for imaging by securing their heads, Applying eye lubricant, and placing a coverslip over the eye. Retinal ganglion cells (RGCs) expressing fluorescent proteins are first visualized using epifluorescence for alignment. Finally, two-photon imaging is employed,...
13:02
Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation
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Cited by 3 •
2017
We present a protocol for fabricating 1-D photonic crystal cavities on subwavelength diameter silica fibers (optical nanofibers) using femtosecond laser-induced ablation.
08:44
Fabrication and Testing of Photonic Thermometers
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Cited by 1 •
2018
We describe the process of fabrication and testing of photonic thermometers.
FAQs
Near-infrared femtosecond laser pulses confine fluorophore excitation to the microscope’s focal point. Regions outside that focal plane therefore receive less direct excitation, helping limit photodamage while investigators observe living tissue. This focal excitation is especially important when imaging deep inside optically scattering specimens, where preserving surrounding tissue supports interpretation of local cellular and vascular responses.
The micropipette provides physical access, while real-time two-photon imaging shows its position relative to the selected cell, vessel, or tissue compartment. This coordination allows investigators to guide the pipette to a specific microscopic target instead of accessing tissue without visual confirmation. The combination supports localized sampling, injection, or manipulation within living specimens.
The approach enables investigators to visualize and access cells, vessels, or tissue compartments located deep within specimens that scatter light. Two-photon imaging supplies focal visualization, and the micropipette acts on the structure selected through that image. Together, these capabilities connect microscopic anatomy with site-specific sampling or intervention rather than relying only on observations near the tissue surface.
Investigators first image the living specimen with two-photon microscopy, identify a cell, vessel, or tissue compartment, and use the real-time image to guide the micropipette to that target. Once positioned, the pipette can support sampling, injection, or localized manipulation. This workflow links visual selection with an immediate, site-specific intervention while the tissue remains observable.
Two-photon Micropuncture can help investigators measure or alter conditions at a selected microscopic site and then relate those changes to functional responses in living tissue. In cancer models, the target may lie within tumor vasculature or the tumor microenvironment. This makes it possible to examine local conditions together with responses to sampling, injection, or manipulation.
In cancer research, the technique supports studies of drug delivery, vascular permeability, tumor cell behavior, and treatment effects in living models. Its value comes from linking microscopic structure with functional responses at selected sites. Investigators can examine tumor vessels or the surrounding microenvironment and assess how localized conditions relate to delivery, permeability, cellular behavior, or therapeutic responses.