Post-synthesis Loading
Post-synthesis Loading - Related Videos
09:42
Solid Phase Synthesis
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2023
Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA Merrifield's solid-phase synthesis is a Nobel Prize winning invention where a reactant molecule is bound on a solid support and undergoes successive chemical reactions to form a desired compound. When the molecules are bound to a solid support, excess reagents and byproducts can be removed by washing away the impurities, while the target compound remains bound to the resin. Specifically, we will...
08:27
Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
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Cited by 10 •
2021
Here, a simple protocol is presented for producing mRNA nanoparticles based on poly(beta aminoester) polymers, easy to be tailored by changing the encapsulated mRNA. The workflow for synthesizing the polymers, the nanoparticles, and their in vitro essential characterization are also described. A proof-of-concept regarding immunization is also added.
09:09
Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
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Cited by 3 •
2016
We present a protocol to synthesize Janus microhydrogels composed entirely of the same base material, poly(N-isopropylacrylamide) (PNIPAAm), with a clearly compartmentalized structure base on the phase separation of a supersaturated NIPAAm monomer solution. The synthesized Janus microhydrogels show unique properties such as anisotropic thermo-responsiveness and organophilic/hydrophilic loading capability.
08:33
Hydrogel Synthesis
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2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
02:36
Transfer RNA Synthesis
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2020
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases. Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
FAQs
Loading efficiency and guest distribution depend on several linked variables. Pore size affects whether a guest can enter, while solvent compatibility influences transport through the host. Guest concentration changes the amount available for uptake, and host–guest interactions determine how strongly molecules, ions, or nanoparticles are retained. Together, these factors influence loading capacity, location, and uniformity.
Diffusion moves guest species through accessible pores, adsorption retains them on internal surfaces, and coordination attaches them to functional groups within the host. These mechanisms can place guests in different environments and create different retention strengths. Consequently, the dominant interaction affects whether the resulting material is better suited to storage, release, sensing, or catalytic use.
Host–guest interactions determine whether an introduced species remains associated with the material after loading. Functional groups can provide coordination sites, whereas internal surfaces can support adsorption. The balance between these interactions and solvent compatibility influences guest retention and accessibility, which are important when the material must release a drug, expose a catalyst, or respond to a sensing target.
A typical workflow starts with a previously synthesized host, such as a porous framework or mesoporous solid, followed by selection of a compatible guest and solvent. The host is then exposed to a chosen guest concentration so diffusion, adsorption, or coordination can occur. Conditions are selected according to pore size and the desired host–guest interaction.
Post-synthesis Loading is useful when researchers want to add catalysts, dyes, drugs, sensing molecules, ions, or nanoparticles without rebuilding the host structure. This separation between host preparation and guest incorporation supports property tailoring after synthesis. It is especially relevant for porous materials whose existing pores and internal functional groups can accommodate additional chemical components.
The resulting materials can combine the structural features of a porous host with the function of an incorporated guest. Examples include catalysts for chemical reactions, dyes or sensing molecules for detection, and drugs for controlled release. Guest incorporation also supports selective separations and multifunctional materials, where several chemical properties are designed within one host system.