Mass Identification
Mass Identification - Related Videos
01:33
Peptide Identification Using Tandem Mass Spectrometry
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2023
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties. This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
10:52
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Cited by 1 •
2017
We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.
13:29
Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
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Cited by 1 •
2014
The application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth expedites the identification of bacteria. The presented method is a rapid and reliable method for identification of Gram negative bacteria directly from blood culture broth.
14:47
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
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Cited by 5 •
2016
MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.
09:07
Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
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Cited by 1 •
2024
EUCAST has developed a direct antimicrobial susceptibility testing (AST) protocol for automated blood cultures. However, its dependence on mass spectrometry-based microbial identification can be obviated by using a direct inoculum preparation protocol in an automated microbial identification system. This approach can provide AST reports within 24 h of sample collection.
FAQs
The mass-to-charge ratio provides the measurement used to separate detected ions within a sample. Because the resulting spectrum records these separated signals, researchers can examine the measured pattern rather than relying only on the original sample composition. This measurement is central to assigning molecular or analyte identities in clinical research.
Identification becomes possible by comparing the observed spectrum with reference spectra or with molecular masses calculated for candidate compounds. Agreement between the measured pattern and an available reference or calculated value supports assignment of the analyte’s identity. This comparison connects an instrumental measurement with a specific molecule, protein, drug, metabolite, or biomarker.
Mass Identification adds molecular mass information to the analysis, helping distinguish and characterize measured analytes more specifically. In clinical research, this can clarify which metabolites, proteins, therapeutic drugs, or disease-associated biomarkers are present. The resulting specificity supports more focused investigation of biological samples and the molecular features associated with disease or treatment.
The approach can characterize several clinically relevant analyte classes, including metabolites, proteins, therapeutic drugs, and disease-associated biomarkers. Examining these different targets allows the same analytical strategy to address biological molecules, treatment-related compounds, and disease-linked signals. The selected analyte category depends on the clinical research question and the sample being investigated.
By identifying metabolites, proteins, and disease-associated biomarkers, Mass Identification can help researchers investigate molecular changes linked with disease. These identifications provide analytical information for studying which measured compounds or biomolecules are associated with pathological processes. The approach therefore supports disease-mechanism research without limiting analysis to a single type of clinical analyte.
A clinical workflow can use measured spectra and their comparison with reference spectra or calculated molecular masses to characterize relevant analytes. Those results may support monitoring of therapeutic drugs or treatment-related changes, while also informing development of laboratory assays. The method is therefore useful both for exploratory clinical research and for refining analytical measurement strategies.