Optic Lobe Imaging
Optic Lobe Imaging - Related Videos
09:00
Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe
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Cited by 37 •
2012
We describe an established technique to measure and analyze odor-evoked calcium responses in the antennal lobe of living Drosophila melanogaster.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
07:23
Hybridization Chain Reaction FISH in the Developing Drosophila Optic Lobe
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2026
This protocol describes the steps required to perform hybridization chain reaction fluorescent in situ hybridization (HCR-FISH) in Drosophila melanogaster larval optic lobes. Procedures for probe design, hybridization, amplification, and tissue mounting are detailed to facilitate visualization of the developing optic lobe.
02:34
Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate
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2023
This video describes a technique to harvest individual lobes from the prostate gland of a mouse model. Individual lobes can then be used for histological analysis and cancer research.
07:46
Optical Materialography Part 2: Image Analysis
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2023
Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA The imaging of microscopic structures of solid materials, and the analysis of the structural components imaged, is known as materialography. Often, we would like to quantify the internal three-dimensional microstructure of a material using only the structural features evidenced by an exposed two-dimensional surface. While X-ray based tomographical methods can reveal buried...
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Optical sections provide views through successive depths of the developing tissue, allowing researchers to assemble a three-dimensional reconstruction rather than relying on a single surface image. This approach reveals the spatial arrangement of cells and tissues, making it possible to examine neural organization, changing architecture, and the emergence of developing visual centers.
Fluorescent markers can distinguish labeled cells or tissues within the optic lobe and show where particular populations are located as development proceeds. By comparing labeled structures across images, researchers can investigate cell proliferation, differentiation, and migration, linking cellular behavior with the formation of organized neural tissue.
Imaging at different developmental stages shows how the optic lobe changes rather than providing only a static description. Researchers can follow the progression from cellular changes to increasingly organized neural architecture, helping them relate cell proliferation, differentiation, migration, and circuit formation to the timing of visual-system development.
Images can be compared across normal and altered developmental conditions to identify changes in neural patterning, organization, or connectivity. These comparisons help researchers assess how genetic programs influence development and how interactions among tissues contribute to optic lobe formation, providing evidence for mechanisms that shape visual centers.
A typical workflow begins by labeling selected cells or tissues with fluorescent markers, collecting microscopy images as optical sections, and examining those sections to reconstruct developing neural architecture. Researchers then compare structures across developmental stages or conditions, using the resulting patterns to evaluate cellular changes, organization, and circuit formation.
Researchers can use the approach when they need to compare normal optic lobe development with altered nervous-system development. Differences in cell arrangement, growth, migration, differentiation, or connectivity may reveal developmental defects and indicate which aspects of neural patterning or tissue organization have been disrupted.
The images support questions about how visual centers form, how neural tissues become patterned, and how developing cells contribute to connectivity and functional visual circuits. In developmental biology, these observations connect cellular behavior with tissue-level organization and help explain how genetic programs and tissue interactions shape the nervous system.