Plant Grafting Technique

Plant grafting is a propagation technique that joins tissues from two plants so they grow as a single, functioning individual. It typically connects a scion, which provides the desired shoot or variety, to a rootstock, which supplies the root system; successful alignment of their cambium layers allows vascular tissues to reconnect and transport water, minerals, and sugars. In environmental and agricultural settings, grafting can combine productive or stress-sensitive plants with rootstocks adapted to drought, salinity, soil-borne pathogens, or poor soils. This approach supports plant establishment, reduces losses, preserves valuable varieties, and may improve resource efficiency under changing environmental conditions.

Plant Grafting Technique - Related Videos

Research

JoVE EoE - Immunotherapy

A Technique for Human Skin Grafting in a Mouse Model

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2025

This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

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Cited by 9 •

2015

A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.

Orthotopic Porcine Kidney Transplantation: A Technique to Transplant Graft Kidney in Porcine Model

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2025

This video describes orthotopic kidney auto-transplantation in a porcine model. This surgical procedure involves the transplantation of an animal's kidney graft at its original anatomical location. The model is used to investigate new treatments for end-stage kidney diseases in humans.

Education

JoVE Core - Biology

Plant Tissue Culture

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2020

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...

Research

JoVE Journal - Medicine
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Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

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Cited by 31 •

2014

Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.

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