Atp Dependent Luminescence
Atp Dependent Luminescence - Related Videos
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
06:16
Phase-Dependent Control of Trap Depth and Persistent Luminescence in Strontium Aluminate Phosphors
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2025
This study investigates the standard optical measurements of two persistent luminescent ceramics: SAO-B (blue) and SAO-G (green), such as thermoluminescence (TL) and persistent luminescence (PersL). By controlling electron trap depth, this work innovates 'temperature-resolved' and 'time-resolved' dynamic anti-counterfeiting methods.
04:00
Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons
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2025
In this video, we describe a luminescence-based method to assess the neurotoxicity of neurite outgrowth-promoting small molecule compounds on the viability of hNPC-derived neurons. The assay measures intracellular ATP level, an indicator of cell viability, to evaluate neurotoxicity following test compound treatment.
10:14
Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
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Cited by 4 •
2014
Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.
FAQs
Comparisons are most meaningful when sampling is standardized and assay conditions are defined consistently. Because emitted light is related to ATP abundance, differences in sampling or assay conditions can affect the signal. Consistent preparation supports interpretation of relative metabolic activity across samples, treatments, or time points more reliably.
The assay couples ATP availability to a light-producing chemical reaction. Luciferase catalyzes oxidation of luciferin in the presence of ATP, oxygen, and cofactors, and the resulting light is quantified. Under defined conditions, this coupling makes the measured signal related to ATP abundance, allowing luminescence to indicate metabolically active biological material.
ATP abundance provides a rapid indicator of metabolically active biological material, so the signal can support estimates of viable microbial burden or host-cell viability. It also enables comparisons of cellular responses between experimental conditions. In infection and immunology studies, this connects a biochemical measurement to changes in microbial presence or the condition of host cells.
A typical workflow begins with standardized sampling, followed by combining the sample with the luciferase-luciferin reaction system under defined conditions. Oxygen, ATP, and required cofactors support the reaction, and emitted light is quantified as the assay signal. Results can then be compared across samples or experimental conditions to evaluate relative biological activity.
Antimicrobial experiments can use the luminescence signal to estimate changes in viable microbial burden after treatment. Signals from differently treated samples are compared under standardized sampling and assay conditions; a change in ATP-associated light indicates a corresponding change in measured metabolically active material. This supports rapid assessment of treatment effects and screening of antimicrobial conditions.
In host-cell experiments, ATP-dependent luminescence provides a rapid readout for comparing cell viability across conditions. Researchers can use the signal to evaluate cytotoxic effects by examining how cellular ATP-associated luminescence changes between experimental groups. This application is useful in immunology research, provided that sampling and assay conditions remain consistent for interpretation.
The method is well suited to screening because light production provides a rapid, sensitive readout across multiple experimental conditions. Process monitoring similarly benefits from repeated comparisons of ATP-associated signals under standardized sampling and assay conditions. These applications still require careful interpretation, because the measured result reflects ATP-related biological activity under the conditions of the assay.