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Biology

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Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
 

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Article DOI: 10.3791/51721
October 25th, 2014

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Summary October 25th, 2014

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

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