Gfp-tagged Mutants
Gfp-tagged Mutants - Related Videos
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
0 Views •
Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
09:14
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
0 Views •
Cited by 5 •
2012
In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.
05:00
Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies
0 Views •
2025
This video demonstrates a method to study the uptake and retrograde transport of functionalized nanobodies to understand their intracellular trafficking pathways. By introducing radiolabelled sulfate and utilizing isolation and detection techniques, this method enables the study of endocytic uptake and trans-Golgi network arrival of cargo proteins.
03:44
Generation of GFP-Tagged Recombinant Respiratory Syncytial Virus Using a T7 Expression System
0 Views •
2026
Source: Bouillier, C. et. al., Amplification, and Titration of Recombinant Respiratory Syncytial Viruses. J. Vis. Exp. (2019)This video demonstrates the generation of a green fluorescent protein (GFP)-expressing recombinant respiratory syncytial virus using a T7-based reverse genetics system. The co-transfection of plasmids encoding viral proteins and a GFP-tagged genome leads to protein expression and initiates virus rescue.
03:12
Visualizing Pathogen-Induced Oxidative Stress in C. elegans Using GFP-Tagged SKN-1
0 Views •
2026
Source: Naji, A., et al. Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans. J. Vis. Exp. (2019)This video demonstrates the use of green fluorescent protein-tagged SKN-1 in C. elegans to monitor oxidative stress by tracking green fluorescence. Worms exposed to S. gordonii show strong nuclear fluorescence, confirming pathogen-induced stress, while those fed E. coli display diffuse green fluorescence in the cytoplasm, indicating minimal SKN-1 activation.
FAQs
The mutation and the fluorescent readout provide different kinds of information. The altered gene or protein supplies the mechanistic change, whereas GFP makes a related signal visible through fluorescence-based detection. A change in localization, abundance, or population behavior can therefore be examined in relation to the selected mutation, helping researchers associate genotype with a specific cellular or microbial phenotype.
When GFP is linked to a protein, microscopy can show where that protein is located rather than only whether the mutant exists. Fluorescence intensity or distribution can also support observations about relative abundance and spatial behavior. This makes the construct useful when the research question concerns how a mutation affects protein placement or presence within cells or microorganisms.
Fluorescence can be interpreted at more than one biological scale. In microscopy, the position of the signal can reveal localization, and its distribution can provide information about abundance. Across a group of microorganisms or cells, fluorescence can instead help track population behavior. Distinguishing these readouts helps researchers match the observed signal to the biological process under investigation.
Detection can be performed by microscopy or by other fluorescence-based methods. Microscopy is especially relevant when the goal is to examine localization or visible behavior, while fluorescence measurements can help follow signal associated with abundance or population behavior. The selected readout depends on which aspect of the mutant system the study seeks to observe.
In infection research, these mutants can help follow pathogen entry and replication while retaining a link to a defined genetic alteration. The fluorescent signal provides a way to observe pathogen-associated behavior, and the mutation helps relate that observation to gene or protein function. This supports analysis of infection-related phenotypes in host-cell systems.
Within immunology, the approach can be used to examine microbial interactions with host cells and assess how selected genes influence immune recognition. It also supports investigation of disease-related phenotypes by connecting an altered gene or protein with an observable fluorescent pattern or population behavior. The same system can therefore address microbial behavior and immune-response questions.