Gene Regulatory Circuits
Gene Regulatory Circuits - Related Videos
07:55
Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes
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Cited by 3 •
2011
A straight-forward and robust method to identify potential regulatory motifs in co-regulated genes is presented. SCOPE does not require any user parameters and returns motifs that represent excellent candidates for regulatory signals. The identification of such regulatory signals helps to understand the underlying biology.
02:02
Cis-regulatory Sequences
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2020
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
12:24
DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
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Cited by 10 •
2014
This video article describes an in vitro microarray based method to determine the gene targets and binding sites for two component system response regulators.
09:07
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Cited by 5 •
2016
In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.
09:27
RC/RL/LC Circuits
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN Capacitors (C), inductors (L), and resistors (R) are each an important circuit element with distinct behaviors. A resistor dissipates energy and obeys Ohm's law, with its voltage proportional to its current. A capacitor stores electrical energy, with its current proportional to the rate of change of its voltage, while an inductor stores magnetic energy, with its voltage...
FAQs
Feedback loops allow regulatory activity to reinforce or restrain itself, helping stabilize a cellular state. Feed-forward loops connect an initial regulator to downstream targets through an additional regulatory step, producing responses that depend on signal timing or context. These circuit structures help explain how cells maintain identity while still adapting to changing conditions.
Promoters and enhancers provide distinct regulatory DNA sites where transcription factors and other molecular regulators influence transcription. Their interactions help determine both whether a gene is activated and how strongly it is expressed. Examining these sites together is important because changes in regulatory control can alter cellular behavior without necessarily changing the gene’s coding sequence.
Abnormal signaling can change the activity of transcription factors or other regulators that control downstream genes. The resulting circuit imbalance may produce inappropriate expression levels or destabilize a normal cellular state. This mechanism connects signaling abnormalities with disease processes, including cancer and immune disease, where altered regulatory responses can affect cell function.
A circuit-based investigation identifies the relevant genes, regulatory DNA regions, and molecular factors, then examines how they influence one another through regulatory interactions. Researchers can compare these relationships with disease-associated mutations, epigenetic changes, or abnormal signaling. The resulting map supports models of disrupted cellular function and helps prioritize mechanisms for further study.
Circuit maps can reveal coordinated regulatory changes associated with a disease state rather than focusing on a single gene in isolation. Such patterns may inform biomarker discovery by linking molecular activity with altered cellular function. They can also identify regulatory points for targeted therapies, while circuit models help anticipate how changing one component may affect related processes.
Development depends on regulatory programs that establish and maintain distinct cellular identities, so circuit disruption can contribute to developmental disorders. Understanding these programs also supports engineered cell systems, in which regulatory relationships may be designed or adjusted to produce desired cellular states. This subject-specific perspective links circuit analysis with both disease explanation and medical biotechnology.