Lung Tissue Harvest
Lung Tissue Harvest - Related Videos
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
06:06
Apparatus for Harvesting Tissue Microcolumns
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Cited by 7 •
2018
Here we describe a protocol for producing harvesting needles that can be used to collect full-thickness skin tissue without causing donor site scarring. The needles can be combined with a simple collection system to achieve high-volume harvesting.
10:20
Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice
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Cited by 17 •
2013
A specific and rapid protocol to simultaneously investigate right heart function, lung inflammation, and the immune response is described as a learning tool. Video and figures describe physiology and microdissection techniques in an organized team-approach that is adaptable to be used for small to large sized studies.
03:03
Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs
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2025
In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.
FAQs
Maintaining lung architecture allows histological analysis to retain the relationships among structures and cells as they existed in the specimen. Preserving cellular components supports cellular studies, while protecting molecular integrity supports molecular analysis. Matching tissue handling and preparation to the intended endpoint helps keep structural, cellular, and molecular findings interpretable.
A representative sample helps ensure that analysis reflects relevant changes within the lung specimen rather than an arbitrary tissue area. This is important when investigators examine infection, inflammation, fibrosis, cancer, or other pulmonary disorders, because the harvested material must provide meaningful structural, cellular, or molecular evidence for the question being studied.
Preparation depends on whether the tissue will undergo diagnostic, cellular, histological, or molecular analysis. Fixation can support preservation for certain examinations, whereas freezing or other preparation methods may be selected for different analytical needs. Choosing an appropriate method helps retain the tissue features required to evaluate architecture, cells, or molecular components.
A typical workflow begins by carefully isolating the relevant lung region and excising representative tissue. The specimen is then directed toward fixation, freezing, or another suitable preparation method based on the planned analysis. Careful control during collection and preparation is important because the final specimen must retain useful structural, cellular, and molecular information.
Harvested lung tissue can support evaluation of infection, inflammation, fibrosis, cancer, and other pulmonary disorders. In research, it also enables investigation of respiratory biology, disease mechanisms, and treatment responses. These uses allow investigators to examine tissue-level evidence while developing a clearer connection between pulmonary changes and therapeutic questions.
In medicine, tissue analysis can connect structural changes in the lung with clinical findings. Histological examination may characterize tissue architecture, while cellular and molecular analyses provide additional layers of evidence. Together, these results can support evaluation of pulmonary disease and contribute to treatment-response studies and therapeutic development.