Pancreatic Graft Preparation
Pancreatic Graft Preparation - Related Videos
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
12:00
Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
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Cited by 3 •
2014
A plasma-induced polymerization procedure is described for the surface-initiated polymerization on polymer membranes. Further postmodification of the grafted polymer with photochromic substances is presented with a protocol of conducting permeability measurements of light-responsive membranes.
10:39
Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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Cited by 8 •
2013
We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.
11:54
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
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Cited by 20 •
2009
Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part I: digestion and collection of pancreatic tissue) using a modified automated method.
10:51
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
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Cited by 31 •
2017
Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.
FAQs
Cooling the donor pancreas in preservation solution helps limit ischemic injury while the organ is outside the recipient’s circulation. This protective step supports preservation of insulin-producing tissue and maintains graft viability until implantation. Its importance lies in reducing injury that could otherwise compromise the organ’s function after transplantation.
Vessel reconstruction prepares the graft for reliable vascular anastomosis during implantation. Careful handling and reconstruction of the pancreatic vessels help establish the connections needed for blood flow after transplantation while limiting mechanical injury. A well-prepared vascular arrangement therefore supports safer implantation and contributes to preservation of graft function.
The pancreatic duct and an attached duodenal segment are preserved to provide a route for drainage from the graft. Maintaining these structures is an essential part of preparation because the transplanted pancreas must retain an anatomical pathway for handling its secretions. Their preservation supports functional integration after implantation.
Ischemic stress and mechanical injury are the main preparation-related threats identified in this process. Prolonged exposure without adequate cooling can impair tissue preservation, while rough inspection, dissection, or reconstruction can physically damage the graft. Careful technique addresses both risks and helps maintain insulin-producing tissue for transplantation.
The workflow begins after procurement with cooling in preservation solution, followed by inspection and dissection of the donor organ. Surgeons then reconstruct the vessels while preserving the pancreatic duct and attached duodenal segment. These steps produce a graft suited for vascular anastomosis and drainage during implantation.
This preparation is especially important when a donor pancreas is being considered for transplantation in a patient with severe diabetes and a need to restore endocrine function. Standardized handling improves transplant quality, supports safer implantation, and may contribute to durable glycemic control by protecting the graft’s insulin-producing tissue.