Pulmonary Block Harvest
Pulmonary Block Harvest - Related Videos
07:06
Selective Harvesting of Marginating-pulmonary Leukocytes
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Cited by 1 •
2016
Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
13:21
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Cited by 6 •
2012
In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.
08:17
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
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Cited by 8 •
2017
The following protocol describes the methodology for the acquisition and analysis of echocardiographic images used to obtain the Left Atrial Volume (LAV), Aorta (Ao) diameter, and Pulmonary Artery (PA) diameter in mice. This technique is a non-invasive, non-terminal procedure that allows assessment of the cardiopulmonary function.
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
FAQs
Limiting ischemic injury helps protect lung tissue during the interval between removal and later transplantation or assessment. The technique therefore emphasizes controlled handling and preservation of tissue integrity while the airway and major vessels are divided. These priorities can influence graft quality and help maintain the lungs in a condition suitable for preparation and implantation.
Maintaining the lungs as one anatomical unit preserves their coordinated relationship with the airway and major blood vessels during procurement. This arrangement can simplify combined assessment and preservation before implantation, especially when both lungs are intended for transplantation. It also allows clinicians to evaluate the pulmonary structures in their original thoracic relationship rather than as isolated components.
Bilateral transplantation requires coordinated preparation of both lungs, making an intact pulmonary unit useful during procurement and assessment. Keeping the organs together can support organized preservation and handling before implantation. The approach also gives clinicians a direct view of how the paired lungs and their shared thoracic structures relate, which is important when planning subsequent preparation.
The workflow begins with exposure of the pulmonary structures and their careful mobilization within the thorax. After the structures are adequately prepared, the airway and major blood vessels are divided while the lungs remain protected as a unit. Attention throughout the sequence centers on maintaining tissue integrity and limiting avoidable ischemic injury during removal.
Clinicians must identify the relationships among the lungs, airway, and major blood vessels before mobilization and division. Understanding these connections supports controlled dissection and helps preserve the structures needed for later assessment or implantation. The technique is therefore also valuable for teaching thoracic anatomy, particularly the spatial organization that affects safe procurement.
In clinical transplantation, the technique provides donor lungs for preservation, preparation, and implantation. In research and training, it offers an intact anatomical model for examining thoracic relationships and the effects of procedural handling on graft quality. These uses connect operative anatomy with transplant practice, helping clinicians relate procurement decisions to potential transplantation outcomes.