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Selective Harvesting of Marginating-pulmonary Leukocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Selective Harvesting of Marginating-pulmonary Leukocytes

Selective Harvesting of Marginating-pulmonary Leukocytes

DOI:
10.3791/53849-v

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07:06 min

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March 11, 2016

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, , ,
1Sagol School of Neuroscience & School of Psychological Sciences,Tel-Aviv University

Chapters

  • 00:05Title
  • 00:55Rat Lung Perfusion and Marginating-Pulmonary (MP)-Leukocyte Collection
  • 02:59Mouse Lung Perfusion and MP-Leukocyte Collection
  • 04:52Results: Representative MP Compartment Leukocyte Subset Composition
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.

Tags

Keywords: Selective HarvestingMarginating-pulmonary LeukocytesLung PerfusionRatPeristaltic PumpButterfly NeedlesHemostatBlood CollectionLung Perfusate

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