Wharton Duct Cannulation
Wharton Duct Cannulation - Related Videos
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
13:17
The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
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Cited by 48 •
2015
Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
04:28
Cannulation of the Carotid Artery in a Rat Model
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2025
In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.
FAQs
Dilation creates an entry path for the fine cannula while helping the operator access the duct through its natural opening beside the lingual frenulum. This step supports subsequent irrigation, contrast injection, stone retrieval, or sialendoscope passage. Careful access is particularly relevant when investigating obstructed salivary flow without immediately resorting to open surgery.
Direct or endoscopic guidance helps the operator locate the ductal papilla and control advancement of the cannula within the duct. Endoscopic access can also support evaluation of strictures and other causes of obstruction. The resulting visualization and duct access connect the cannulation step with both diagnostic assessment and minimally invasive treatment of salivary duct disease.
Access may help evaluate strictures, stones, and other causes of obstructed salivary flow. These findings are relevant to obstructive sialadenitis, in which impaired drainage affects the submandibular gland. By creating a route for inspection, contrast injection, or therapeutic maneuvers, the technique links the physical duct abnormality with the clinical problem being investigated.
The procedure begins by locating the ductal papilla beside the lingual frenulum. The opening is then gently dilated, followed by advancement of a fine cannula into Wharton duct under direct or endoscopic guidance. Once access is established, the channel can support irrigation, contrast injection, stone retrieval, or passage of a sialendoscope, depending on the clinical purpose.
The cannula can provide a route for irrigation or contrast injection and may support stone retrieval. It can also permit passage of a sialendoscope, an instrument used to evaluate the duct. These options allow the same access route to serve diagnostic and therapeutic goals, including assessment of strictures and other causes of impaired salivary drainage.
Wharton Duct Cannulation is useful when clinicians need minimally invasive access to investigate or treat obstructive sialadenitis and related salivary duct obstruction. By supporting endoscopic evaluation, irrigation, contrast studies, or stone retrieval, it may help preserve submandibular gland function while reducing the need for open surgery. Its value therefore extends beyond access alone to gland-preserving management.