Led Crosslinking
Led Crosslinking - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
07:40
Using Affordable LED Arrays for Photo-Stimulation of Neurons
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Cited by 7 •
2011
Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
07:24
Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal
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2025
This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.
FAQs
The photoinitiator acts as the light-responsive trigger for the reaction. When it absorbs the LED’s delivered wavelength, it generates reactive species that start polymerization or create links between polymer chains. Its presence connects the optical input to material transformation, allowing researchers to convert a light-sensitive formulation into a structured scaffold or matrix under controlled exposure.
Wavelength must correspond to light absorbed by the photoinitiator so the LED can generate the reactive species required for polymerization or crosslink formation. Exposure conditions also determine where and when the reaction occurs. Controlling these variables supports localized activation and helps researchers tune the resulting material structure, shape, and mechanical properties for experimental use.
Localized activation allows material modification to occur in selected regions rather than throughout the entire sample. This spatial control is valuable when researchers need defined shapes or engineered microenvironments with different structural features. In neuroscience studies, such precision can help organize hydrogel scaffolds and cell-encapsulation matrices for examining how neurons respond to their surroundings.
A typical workflow begins with a light-sensitive material containing a suitable photoinitiator, followed by positioning the material for controlled LED exposure. The LED supplies the relevant wavelength, the photoinitiator generates reactive species, and polymer chains become linked as polymerization or crosslinking proceeds. The resulting structure can then serve as a shaped scaffold, model, or matrix.
Researchers can apply Led Crosslinking when they need hydrogel scaffolds, neural tissue models, or matrices that encapsulate cells while providing defined shapes and mechanical properties. These engineered environments support investigations of neuronal growth and signaling. The method is also relevant to tissue-repair research because localized, tunable fabrication can create experimental settings that mimic selected structural features.
The process can produce engineered microenvironments whose geometry and mechanical properties are deliberately specified. Those features give researchers a framework for studying neuronal growth, signaling, and tissue repair in a controlled setting. By adjusting where activation occurs and how the material is structured, experiments can compare cellular responses across differently designed neural models or scaffolds.