Plantar Punch Biopsy
Plantar Punch Biopsy - Related Videos
02:20
Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy
0 Views •
2025
This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.
06:19
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
0 Views •
Cited by 163 •
2013
The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
0 Views •
2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
05:26
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
0 Views •
Cited by 3 •
2022
The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research laboratories.
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
0 Views •
Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
FAQs
The full-thickness sample preserves the dermal-epidermal junction, the anatomical boundary used for fiber counting. Investigators identify nerve fibers that cross from the dermis into the epidermis and report their number as intraepidermal nerve fiber density. This converts microscopic nerve organization into an objective measure of small-fiber integrity for neuroscience research.
Protein gene product 9.5, commonly called PGP 9.5 in the protocol, is used during immunostaining to make the relevant nerve fibers identifiable in tissue sections. After staining, researchers can examine fibers at the dermal-epidermal junction and count those entering the epidermis. The resulting density provides a microscopic readout rather than a purely qualitative visual impression.
Comparing samples from different skin sites can show whether small-fiber measurements vary by location, while repeated sampling over time can reveal changes associated with disease or treatment. These comparisons are important because the biopsy does not merely provide a single image; it supports structured assessment of spatial patterns and longitudinal change in cutaneous nerve fiber integrity.
The workflow starts with local anesthesia, followed by removal of a circular, full-thickness skin sample with a punch. The specimen is then fixed, cut into sections, and immunostained, commonly using protein gene product 9.5. Researchers examine the prepared tissue and count intraepidermal nerve fibers crossing the dermal-epidermal junction to obtain the measurement.
The measurement can support studies of peripheral neuropathy, pain, and neurodegenerative disorders. By providing an objective indicator of small-fiber integrity, it helps researchers characterize how these conditions relate to cutaneous sensory nerves. It can also be used when evaluating treatment-related changes, especially when measurements are compared across sites or collected over time.
For peripheral neuropathy research, the measured density offers a tissue-based way to examine small-fiber integrity in human skin. Researchers can relate this objective indicator to disease characterization and then compare values across anatomical sites or over time. Those comparisons may also help identify treatment-related changes in the cutaneous nerve fiber measurement.