R-camp Neurons
R-camp Neurons - Related Videos
06:03
Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ
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Cited by 1 •
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The protocol presented in this study illustrates the effectiveness of the cAMP Difference Detector In Situ in measuring cAMP through two methods. One method utilizes a 96-well plate reading spectrophotometer with HEK-293 cells. The other method demonstrates individual HASM cells under a fluorescent microscope.
01:25
cAMP-dependent Protein Kinase Pathways
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2023
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
07:36
Mechanical Ventilation Boot Camp Curriculum
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Cited by 3 •
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This curriculum outlines how to execute a simulation-based boot camp to teach providers how to manage mechanically ventilated patients.
03:32
Using a Macrophage Conditioned Medium to Promote Neuron Extensions
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2025
This video demonstrates an assay for promoting the growth of neuron projections known as neurite outgrowth using the macrophage-conditioned medium. Co-culture neurons and macrophages with cAMP and harvest the macrophage-derived conditioned medium. Growing the neurons with the conditioned medium promotes neurite outgrowths. These outgrowths are fluorescently labeled and visualized using a fluorescence microscope.
05:44
Developing a Neuron and Macrophage Coculture In Vitro
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2025
This video shows the co-culturing of murine dorsal root ganglion neurons and macrophages. Neurons are seeded on a coated plate, while macrophages are collected from an euthanized mouse, treated, and added to the culture insert. Treatment with db-cAMP activates neurons and prompts macrophages to adopt a pro-regenerative phenotype to support neuronal outgrowth.
FAQs
Neuronal firing opens voltage-gated calcium channels, allowing intracellular calcium levels to rise. Calcium then binds R-CaMP, producing a change in red fluorescence. This sequence links electrical activity to an optical signal, so researchers can monitor activity without relying exclusively on direct electrode-based measurements.
Voltage-gated calcium channels provide the connection between neuronal firing and the indicator response. Their opening permits calcium to enter the neuron, and the resulting intracellular increase activates the fluorescence change detected from R-CaMP. Without this activity-linked calcium step, fluorescence would not provide the same readout of neural firing.
Fluorescence changes provide an optical readout that researchers can use to follow activity in individual neurons or across neural populations. Examining these signals over defined circuits helps reveal patterns of activity rather than only isolated cellular events, supporting investigations of how groups of neurons participate in sensory processing and behavior.
A basic workflow uses neurons that express R-CaMP, observes them during live imaging, and records changes in red fluorescence as intracellular calcium changes. Researchers can analyze signals from single cells or neural populations and then relate the observed activity patterns to the circuit, sensory condition, behavior, or disease model under study.
Researchers use this approach when they need to connect neural activity with sensory processing or behavior in defined circuits. Live imaging allows activity patterns to be followed while focusing on individual neurons or populations, making it possible to examine how circuit-level signals correspond to the sensory or behavioral context being investigated.
R-CaMP neurons provide an optical alternative for monitoring activity, which can reduce reliance on electrode-based recordings. Their value lies in observing individual cells and neural populations within defined circuits through fluorescence changes linked to calcium. This supports circuit-level studies of sensory processing, behavior, and disease models using a complementary measurement strategy.