1. Preparation Materials commonly used include 70% alcohol in a squirt bottle, sterile 20-25 gauge needles, two packs of sterile instruments of iris scissors and thumb forceps (one for the skin and one for the internal tissues), paper towels, sterile surgeon gloves, a sterile petri dish, sterile medium, and sterile saline. The hood fan should be running for at least 15 minutes prior to the procedure, and it should be disinfected with 70% alcohol. If the hood is equipped with a UV steriliza…