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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
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December 13, 2013
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The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.
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Ivanova, E., Toychiev, A. H., Yee, C. W., Sagdullaev, B. T. Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry. J. Vis. Exp. (82), e51018, doi:10.3791/51018 (2013).
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