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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

37,432 Views

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10:35 min

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September 06, 2014

DOI:

10.3791/51682-v

DOI:
10.3791/51682-v

•

10:35 min
September 06, 2014

•

77 Views
, , , , ,
1Division of Cardiothoracic Surgery,Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital and Department of Anesthesia, Harvard Medical School

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A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

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Preble, J. M., Pacak, C. A., Kondo, H., MacKay, A. A., Cowan, D. B., McCully, J. D. Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration. J. Vis. Exp. (91), e51682, doi:10.3791/51682 (2014).

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