Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Robert A. Hill; Jelena Medved; Kiran D. Patel; Akiko Nishiyama

doi:10.3791/51835

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Nörobilim

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Organotipik Dilim Kültürler Oligodendrosit Dinamikler ve miyelinasyonun incelemek için

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

JoVE Journal
Nörobilim

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JoVE Journal Nörobilim

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Organotipik Dilim Kültürler Oligodendrosit Dinamikler ve miyelinasyonun incelemek için

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18,249 Views

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09:45 min

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August 25, 2014

DOI:

10.3791/51835-v

DOI:

10.3791/51835-v

•

09:45 min

August 25, 2014

•

12 Views

Robert A. Hill^1,3, Jelena Medved¹, Kiran D. Patel¹, Akiko Nishiyama^1,2

¹Department of Physiology and Neurobiology,University of Connecticut, ²Stem Cell Institute,University of Connecticut, ³Department of Neurology,Yale University School of Medicine

Özet

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.