Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Krzysztof Bobik; John R. Dunlap; Tessa M. Burch-Smith

doi:10.3791/51844

Dergi

/

Biyoloji

/

Tandem Yüksek basınçlı Donma ve Transmisyon Elektron Mikroskobu için Bitki Doku Hızlı Dondurma Değişiklik

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

JoVE Journal
Biyoloji

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal Biyoloji

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Tandem Yüksek basınçlı Donma ve Transmisyon Elektron Mikroskobu için Bitki Doku Hızlı Dondurma Değişiklik

Please note that all translations are automatically generated. Click here for the English version.

16,324 Views

•

12:52 min

•

October 13, 2014

DOI:

10.3791/51844-v

DOI:

10.3791/51844-v

•

12:52 min

October 13, 2014

•

2 Views

Krzysztof Bobik¹, John R. Dunlap², Tessa M. Burch-Smith¹

¹Department of Biochemical, Cellular and Molecular Biology,University of Tennessee, Knoxville, ²Advanced Microscopy and Imaging Facility,University of Tennessee, Knoxville

Özet

Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.