A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Michael J. Rothrock Jr.; Kelli L. Hiett; John Gamble; Andrew C. Caudill; Kellie M. Cicconi-Hogan; J. Gregory Caporaso

doi:10.3791/52161

Dergi

/

Biyoloji

/

Kanatlı Üretim Örneklerden Bakteriyel Topluluklar Nitel ve Nicel Değerlendirme Karma DNA Ekstraksiyon Yöntemi

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

JoVE Journal
Biyoloji

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal Biyoloji

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Kanatlı Üretim Örneklerden Bakteriyel Topluluklar Nitel ve Nicel Değerlendirme Karma DNA Ekstraksiyon Yöntemi

Please note that all translations are automatically generated. Click here for the English version.

14,453 Views

•

07:24 min

•

December 10, 2014

DOI:

10.3791/52161-v

DOI:

10.3791/52161-v

•

07:24 min

December 10, 2014

•

1 Views

Michael J. Rothrock Jr.¹, Kelli L. Hiett², John Gamble³, Andrew C. Caudill⁴, Kellie M. Cicconi-Hogan¹, J. Gregory Caporaso⁵

¹Egg Safety and Quality Research Unit,USDA-Agricultural Research Service, ²Poultry Microbiological Safety and Processing Research Unit,USDA-Agricultural Research Service, ³Department of Biochemistry and Biophysics,Oregon State University, ⁴College of Public Health,University of Georgia, ⁵Department of Biological Sciences, Center for Microbial Genetics and Genomics,Northern Arizona University

Özet

A novel semi-automated hybrid DNA extraction method for use with environmental poultry production samples was developed and demonstrated improvements over a common mechanical and enzymatic extraction method in terms of the quantitative and qualitative estimates of the total bacterial communities.