Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

Jared M. Schrader; Lucy Shapiro

doi:10.3791/52633

Dergi

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Biyoloji

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Senkronizasyon Caulobacter crescentus Bakteriyel Hücre Döngüsü Soruşturma

Synchronization of <em>Caulobacter Crescentus</em> for Investigation of the Bacterial Cell Cycle

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

Senkronizasyon Caulobacter crescentus Bakteriyel Hücre Döngüsü Soruşturma

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11,852 Views

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08:02 min

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April 08, 2015

DOI:

10.3791/52633-v

DOI:

10.3791/52633-v

•

08:02 min

April 08, 2015

•

11836 Views

Jared M. Schrader¹, Lucy Shapiro¹

¹Department of Developmental Biology,Stanford University School of Medicine

Özet

Synchronization of bacterial cells is essential for studies of the bacterial cell cycle and development. Caulobacter crescentus is synchronizable through density centrifugation allowing a rapid and powerful tool for studies of the bacterial cell cycle. Here we provide a detailed protocol for the synchronization of Caulobacter cells.