Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Sarah J. Smith; Eric J. Horstick; Ann E. Davidson; James Dowling

doi:10.3791/53183

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Gelişim Biyolojisi

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Evans Blue Dye ile Zebra balığı Larva İskelet Kası Bütünlüğü Analizi

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

JoVE Journal
Gelişim Biyolojisi

This content is Free Access.

JoVE Journal Gelişim Biyolojisi

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Evans Blue Dye ile Zebra balığı Larva İskelet Kası Bütünlüğü Analizi

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9,082 Views

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07:34 min

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November 30, 2015

DOI:

10.3791/53183-v

DOI:

10.3791/53183-v

•

07:34 min

November 30, 2015

•

11 Views

Sarah J. Smith*^1,2, Eric J. Horstick*^3,4, Ann E. Davidson^1,2, James Dowling^1,2,4

¹Program in Genetics & Genome Biology,The Hospital for Sick Children, ²Department of Molecular Genetics,The University of Toronto, ³Program in Genomics of Differentiation,Eunice Kennedy Shriver National Institute of Child Health and Human Development, ⁴Departments of Pediatrics and Neurology,University of Michigan

Özet

In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.