<em>In Vivo</em> Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

Wei Ding; Michelle E. Bishop; Lascelles E. Lyn-Cook; Kelly J. Davis; Mugimane G. Manjanatha

doi:10.3791/53833

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Sıçan Karaciğer DNA Strand Breaks ve Oksidatif DNA Hasar Ölçüm Vivo Alkali Comet Assay ve Enzim-modifiye Alkali Comet Tahlili

<em>In Vivo</em> Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

Sıçan Karaciğer DNA Strand Breaks ve Oksidatif DNA Hasar Ölçüm Vivo Alkali Comet Assay ve Enzim-modifiye Alkali Comet Tahlili

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15,317 Views

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10:38 min

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May 04, 2016

DOI:

10.3791/53833-v

DOI:

10.3791/53833-v

•

10:38 min

May 04, 2016

•

3 Views

Wei Ding¹, Michelle E. Bishop¹, Lascelles E. Lyn-Cook¹, Kelly J. Davis², Mugimane G. Manjanatha¹

¹Division of Genetic and Molecular Toxicology,US FDA/National Center for Toxicological Research, ²Toxicologic Pathology Associates,US FDA/National Center for Toxicological Research

DEŞİFRE METNİ

Özet

The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.