Floresan Aktif Hücre Sıralama (FACS) ve Taze ve Dondurulmuş Rat Beyin Dokusu gelen Fos-ifade nöronlar Gen İfadesi Analizi
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August 27, 2016
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Burada taze ve dondurulmuş hem de beyin dokusundan nöronal topluluklarından Fos-ifade moleküler değişiklikler incelemek için bir Floresan Aktif Hücre Sıralama (FACS) protokol mevcut. dondurulmuş doku kullanımı değerli hayvan deneklerin kullanımını maksimize için birden fazla seans boyunca birçok beyin bölgelerinin FACS izolasyonu sağlar.
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Cite this Article
Rubio, F. J., Li, X., Liu, Q., Cimbro, R., Hope, B. T. Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue. J. Vis. Exp. (114), e54358, doi:10.3791/54358 (2016).
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