Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Gatikrushna Singh; Sarah M. Fritz; Arnaz Ranji; Deepali Singh; Kathleen Boris-Lawrie

doi:10.3791/54391

Dergi

/

Biyokimya

/

Oligonükleotid yönelik elüsyon kullanılarak Hücreler Aynı Kökenli RNA-protein kompleksleri izolasyonu

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

JoVE Journal
Biyokimya

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal Biyokimya

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Oligonükleotid yönelik elüsyon kullanılarak Hücreler Aynı Kökenli RNA-protein kompleksleri izolasyonu

Please note that all translations are automatically generated. Click here for the English version.

9,017 Views

•

10:53 min

•

January 16, 2017

DOI:

10.3791/54391-v

DOI:

10.3791/54391-v

•

10:53 min

January 16, 2017

•

8996 Views

Gatikrushna Singh*¹, Sarah M. Fritz*², Arnaz Ranji², Deepali Singh³, Kathleen Boris-Lawrie^1,2

¹Department of Veterinary & Biomedical Sciences,University of Minnesota ²Department of Veterinary Biosciences,Ohio State University ³School of Biotechnology,Gautam Buddha University

Özet

This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.