Sistein Thiols rekombinant proteinler Vitro siteye özgü Glikozilasyon için hedefleme
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October 04, 2017
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Biyokimyasal ve yapısal analizleri glikozile proteinlerin homojen örnekleri nispeten büyük miktarda gerektirir. Burada, reaktif Cys thiols hedefleyerek bakterilerden saflaştırılmış rekombinant proteinlerin siteye özgü Glikozilasyon için verimli bir kimyasal yöntem mevcut.
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Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).
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