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CRISPR-aracılı kaybı serebellar granül işlev analizinin gen transferi Elektroporasyon tabanlı rahim içinde kullanarak hücreleri
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using <em>In Utero</em> Electroporation-based Gene Transfer
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JoVE Journal Gelişim Biyolojisi
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

CRISPR-aracılı kaybı serebellar granül işlev analizinin gen transferi Elektroporasyon tabanlı rahim içinde kullanarak hücreleri

Please note that all translations are automatically generated. Click here for the English version.
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9,823 Views

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09:39 min

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June 09, 2018

DOI:

10.3791/57311-v

DOI:
10.3791/57311-v

•

09:39 min
June 09, 2018

•

9785 Views
, , , , ,
1Division of Molecular Neurogenetics,German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance2Division of Molecular Genetics,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK)3Hopp-Children’s Cancer Center at the NCT Heidelberg (KiTZ)4Division of Pediatric Neurooncology,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK)5Department of Pediatric Hematology and Oncology,Heidelberg University Hospital

Özet

Automatically generated

Geleneksel işlev kaybı çalışmalar nakavt hayvanlar kullanarak genlerin kez pahalı ve zaman alıcı olmuştur. Somatik mutagenesis CRISPR-aracılı Elektroporasyon tabanlı gen içinde vivoişlevleri anlamak için güçlü bir araçtır. Burada, nakavt fenotipleri beyincik Proliferasyona hücrelerdeki analiz etmek için bir yöntem raporu.

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Cite this Article

Feng, W., Herbst, L., Lichter, P., Pfister, S. M., Liu, H., Kawauchi, D. CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. J. Vis. Exp. (136), e57311, doi:10.3791/57311 (2018).

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