CRISPR Kılavuzu RNA memeli sistemler için klonlama
77.2K görüntülenme
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16 kez atıf aldı
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06:48 dk.
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2 Ekim 2018
DOI :10.3791/57998-v
2 Ekim 2018
•77.2K görüntülenme
Sathiji Nageshwaran*1,2 , Alejandro Chavez*1,2,3 , Nan Cher Yeo1,2 , Xiaoge Guo1,2 , Alissa Lance-Byrne1 , Angela Tung1 , James J. Collins1,4,5,6,7 , George M. Church1,2
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute
* These authors contributed equally
Burada, bir basit, verimli ve sgRNA klonlama düşük maliyetli yöntemi anlattı.
Chapters in this video
0:04
Title
0:25
Preparation of the Primers and Expression Vectors
2:05
Ligating the Annealed gRNA Oligonucleotides
2:59
Transformation of E. coli
4:03
PCR Analysis
5:35
Results: CRISPR Guided RNA Cloning
6:23
Conclusion
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