İfade ve insan Lipid duyarlı ba * kanal TRPC3 arınma için tek-parçacık Cryo-elektron mikroskobu ile yapısal kararlılık
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January 07, 2019
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Bu iletişim kuralı tarafından cryo-elektron mikroskobu, verimli bir şekilde en az çaba ve toksisite, protein ayıklama, arıtma ile memeli hücrelerinde genlerin ifade etmek için kullanılan bir baculovirus sistemi de dahil olmak üzere iyon kanal yapılarını belirlemek için kullanılan teknikler açıklanır, ve kalite kontrol, örnek kılavuz hazırlık ve tarama, hem de veri toplama ve işleme.
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Haley, E., Choi, W., Fan, C., Sun, W., Du, J., Lü, W. Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy. J. Vis. Exp. (143), e58754, doi:10.3791/58754 (2019).
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