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Purification of the Dendritic Filopodia-rich Fraction
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Purification of the Dendritic Filopodia-rich Fraction

Purification of the Dendritic Filopodia-rich Fraction

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11:51 min

May 02, 2019

DOI:

11:51 min
May 02, 2019

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Özet

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In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

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